Single channel pipettes, fixed volume, MiniPipettes
Supplier: EDVOTEK
Micropipettes which use standard pipette tips. No need to calibrate and near impossible to measure the wrong volume.
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Kits: analysis of Eco RI cleavage patterns of lambda DNA, Ready-to-Load™
Supplier: EDVOTEK
Ready-to-Load™ kit for introducing the use of restriction enzymes as a tool to digest lambda DNA at specific nucleotide sequences.
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Experimentation kits, introduction to plant cell culture
Supplier: EDVOTEK
Genetic modification of plants is a controversial area of biotechnology. All such experiments in plants begin with establishing plant cells in culture. This involves de-differentiating plant cells to form plant 'stem cells'. In this experiment, students establish cell cultures of African violets from leaves. They then use plant growth regulators to encourage root growth from the cultured cells, and produce a mature plant.
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Experimentation kits, PCR-based Alu-Human DNA typing
Supplier: EDVOTEK
Students use primers for a 300 base pair Alu insertion in chromosome 16 (PV92) to determine their own genotype. They can then compare their class results with others around the world over the internet.
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Experimentation kits, blood typing
Supplier: EDVOTEK
ABO and Rh typing of blood left at the scene of a crime can help to narrow down a list of suspects. In this experiment, students use agglutination to identify the blood group of unknown blood samples as a step to identify a criminal.
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Experimentation kits, analysis and comparision of mammalian cell types
Supplier: EDVOTEK
Observe the differences between mammalian cell types and how these cells funciton. Cells are fixed on microscope slides and students stain the cells on the slide to view morphological characteristics of the cell types. These cells are very safe for classroom use.
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Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase
Supplier: EDVOTEK
When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.
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Experimentation kits, in search of the 'Kissing Disease'
Supplier: EDVOTEK
Infectious mononucleosis is commonly known as the 'kissing disease'. The causative agent is Epstein-Barr virus (EBV), which can be transmitted through saliva during kissing. In this experiment, students search for the presence of EBV using the ELISA reaction to detect specific viral proteins.
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Kits: in search of the cancer gene, Ready-to-Load™
Supplier: EDVOTEK
Suppressor genes, such as p53, are essential for cell functions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations of genes can either be inherited or accumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation.
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Experimentation kits, water quality testing I: chromogenic analysis of water bacteria contaminants
Supplier: EDVOTEK
Safe drinking water is vitally important to health. Both pathogenic and harmless bacteria can be found in the guts of mammals and birds. Testing water for every possible type of pathogenic bacteria is slow and costly. Thus, water is tested for a characteristic type of gut bacteria - the coliforms - including the familiar E. coli. Presence of coliforms is an indicator for faecal contamination. In this experiment, students test for coliforms in simulated contaminated water using colour and fluorescent reagents. They can use these same reagents to test water samples from the environment. As an extension activity, a Gram Stain test can be performed on the collected samples.
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M12 Ultra™ Electrophoresis and Visualisation Package
Supplier: EDVOTEK
Our best-selling M12 Complete™ just got even better. The M12 Ultra™ includes the M12 Complete™ electrophoresis package and the all-new TruBlu™ Jr. Blue light transilluminator. This dynamic duo enables real-time visualisation of SYBR® Safe or other blue light-reactive dye agarose gels.
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Safety spectacles, Laboratory
Supplier: EDVOTEK
Practical, durable safety spectacles offering good protection and comfort.
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Experimentation kits, principles of gel filtration chromatography
Supplier: EDVOTEK
This experiment introduces chromatographic separation to the class and shows students how dyes of different colours separate on the basis of their size and shape. Columns may be rinsed and reused.
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Kits: amplification of DNA for fingerprinting
Supplier: EDVOTEK
Ready-to-Load™ kit for teaching DNA fingerprinting and to show how DNA evidence is used in modern forensics. This experiment allows for varied results depending on the selection of DNA fingerprinting patterns.
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Power source, TetraSource™ 300
Supplier: EDVOTEK
Designed to power four M6 or M12 units, or two HexGels units at one time, the TetraSource™ 300 features an easy-to-use, fully programmable, interface for setting voltage, current or timer control with each parameter displayed in real-time. Programmes can be paused or resumed at any point.
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What does DNA look like?
Supplier: EDVOTEK
This Lab activity shows students what real chromosomal DNA Looks Like and allows them to explore the procedures involved in DNA extraction.
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Experimentation kits, mitochondrial DNA analysis using PCR
Supplier: EDVOTEK
The mitochondria are thought to have evolved from a symbiotic relationship between prokaryotic and eukaryotic cells. Mitochondria have their own DNA and are only inherited via the maternal line. In this experiment, students amplify two regions of their mitochondrial DNA.
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Experimentation kits, purification of the restriction enzyme Eco RI
Supplier: EDVOTEK
In this experiment, students purify the restriction enzyme, Eco RI. This procedure utilised an ion exchange chromatograph step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.
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Kits: detection of mad cow disease, Ready-to-Load™
Supplier: EDVOTEK
Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.
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Experimentation kits, molecular weight determination of proteins (agarose-based)
Supplier: EDVOTEK
This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.
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PCR beads
Supplier: EDVOTEK
Each PCR Bead contains Taq DNA polymerase, Taq DNA polymerase buffer, dNTP mixture and MgCl₂. Pack contains sufficient for 25 reactions.
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ReadyPour™ Luria Broth (LB) agar with ampicillin
Supplier: EDVOTEK
Educational Materials, Microbiology, Application: Microbiology, ReadyPour™ LB agar with ampicillin
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Melt & Pour UltraSpec-Agarose™
Supplier: EDVOTEK
0.8% UltraSpec-Agarose™ prepared with TAE buffer. Simply melt, cool and pour.
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Long wave UV Mini-Light
Supplier: EDVOTEK
A safe, simple to use, battery-operated portable mini long wave UV light.
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Experimentation kits, isolation of E. coli Chromosomal DNA
Supplier: EDVOTEK
Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.
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Vortex mixer, Tornado™ Vortexer
Supplier: EDVOTEK
Compact vortex mixer that will accommodate single tubes or a whole handful at once. Two modes, 'touch' or continuous operation, make this ideal for any experiments that require vigorous mixing.
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Kits: in search of the sickle cell gene by southern blot, Ready-to-Load™
Supplier: EDVOTEK
Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.