Supplier: Cytiva

The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

Supplier: Cytiva

His Mag Sepharose® Ni are magnetic beads designed for rapid, small-scale purification and screening of histidine-tagged proteins from different sources.

Supplier: Cytiva

Blotting paper, Sheets, 210×260 mm

Supplier: Cytiva

Cytopore1 macroporous microcarriers are principally designed for use in suspension culture systems for growth of adherent recombinant Chinese Hamster Ovary (CHO) cells and the production of recombinant proteins.

Supplier: Cytiva

Cytopore 2 macroporous microcarriers are principally designed for use in suspension culture systems for growth of adherent diploid cells requiering a higher surface charge and the production of recombinant proteins.

Supplier: Cytiva

Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

Supplier: Cytiva

HiLoad™ Superdex™ prep grade are prepacked XK columns designed for pre-parative gel filtration. Superdex™ prep grade is a composite matrix of dextran and highly cross-linked agarose. The steep selectivity of dextran, and the high chemical and physical stability of agarose, enable high resolution separations. The chromatography media combines high mechanical strength with high hydrophilicity, allowing high flow rates and minimal non specific interactions.

Supplier: Cytiva

Column type: FPLC column, HiPrep™ Capto Q column, Bed dimensions: 16×100 mm

Supplier: Cytiva

Filter cartridge, Type: Female luer-lock to female luer-lock adapter, nylon

Supplier: Cytiva

Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

Supplier: Cytiva

Microfraction collection kit, For: Fraction collector, Frac-950

Supplier: Cytiva

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

Supplier: Cytiva

HiTrap™ Capto™ Q ImpRes is a strong anion exchanger prepacked with BioProcess Capto™ ImpRes media for screening and small-scale protein purifications using ion exchange chromatography (IEX).

Supplier: Cytiva

INdEX™ columns are easy-to-use, general purpose, glass columns well-suited for applications such as process development and diagnostics production. These columns are characterised by their simple design and the novel, axial compression packing method that yields a densely packed bed in under 10 minutes.

Supplier: Cytiva

illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

Supplier: Cytiva

Autoradiography film, Photographic step tablet

Supplier: Cytiva

The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.

Supplier: Cytiva

Column package includes affinity chromatography and size exclusion chromatography columns to achieve very high purity of his-tagged proteins using a 2-step purification protocol.

Supplier: Cytiva

The simple, modular design of miniVE vertical electrophoresis system consolidates all the small parts for electrophoresis and blotting into one convenient unit. Uniform gel temperature is maintained with the tank-style lower buffer chamber. A full tank stabilises the temperature so that lanes are straight and bands are flat. The blot module will accept two gel/membrane stacks simultaneously. The lower buffer chamber is designed to hold two blot modules, allowing four gels to be blotted at once.

Supplier: Cytiva

Agarose NA has been prepared to give the optimal combination of these properties: Reliable separation of nucleic acids; low inhibition of enzyme activity on recovered material; and high gel strength.

Supplier: Cytiva

Clamp for column holder, Clamp for column holder, For: ÄKTAprime

Supplier: Cytiva

Yeast protein extraction buffer kit is a mild detergent-based cell lysis method developed for total extraction of soluble proteins from yeast cells. This kit eliminates the need for glass beads, a common method of mechanical lysis for yeast cells, and is a proprietary improvement on the Zymolyase®-based method for spheroplast preparation and extraction of soluble proteins from yeast cells.

Supplier: Cytiva

HiTrap Capto MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto MMC ImpRes weak cation exchange multimodal resin for screening and small-scale protein purifications using ion exchange chromatography (IEX).

Supplier: Cytiva

Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.

Supplier: Cytiva

HiTrap™ Protein A HP is a pre-packed, ready to use Protein A Sepharose™ High Performance column for preparative purification of monoclonal and polyclonal antibodies from ascites, serum and cell culture supernatants.

Supplier: Cytiva

Amersham ECL Plex™ Western blotting combination packs offer a complete system for fluorescent Western blotting, with improved formulation Cy3 conjugates for Lower background.

Supplier: Cytiva

Convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel, High desalting capacity, The use of centrifugation protocol with all gravity columns.

Supplier: Cytiva

HiTrap MabSelect SuRe columns are prepacked with MabSelect SuRe, a BioProcess resin for purification of monoclonal antibodies from large sample volumes.

Supplier: Cytiva

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Supplier: Cytiva

ECL Plex Cy2-, Cy3-, and Cy5-conjugated Antibodies are part of an optimized system for quantitative analysis using fluorescent Western blotting.