56 Results for: "Amino Acids & Media Supplements"

Supplier: MP Biomedicals

DOB is a component of Synthetic Defined Media (SD), which is used for growth of S. cerevisiae.

Supplier: STEMCELL Technologies

For hydrolysis of hyaluronic acid.

Supplier: HyClone products (Cytiva)

HyClone™ ActiPro™ cell culture media has been formulated to provide high yields of recombinant proteins in bioprocesses using Chinese hamster ovary (CHO) cell lines. The cultured medium is chemically defined, animal-derived component-free (ADCF), and optimized for high-yield protein expression and production in batch or CHO fed-batch cell culture processes.

Supplier: STEMCELL Technologies

For digestion of native elastin.

Supplier: Corning

Corning® Nu-Serum™ growth media supplement provides a low-protein alternative to newborn calf, foetal bovine, and other sera routinely used for cell culture. The low-protein content facilitates protein purification, virus production, monoclonal antibody production and screening, and increases the frequency of successful transfection of cells.

Supplier: PROVITRO

The supplement kit for endothelial cell proliferation medium is suitable for culturing Provitro human endothelial cells after adding the supplement kit components to 500 ml basal medium The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. The supplement kit for endothelial cell priliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time.

Supplier: STEMCELL Technologies

Animal component-free non-specific protease.

Supplier: MP Biomedicals

RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. As a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.

Supplier: STEMCELL Technologies

Collagenase/dispase blend with high collagenase and high caseinase activity.

Supplier: MP Biomedicals

Pyridoxal hydrochloride is used for labeling amino acids and for their detection in the picomole range.

Supplier: STEMCELL Technologies

Collagenase/dispase blend with high collagenase and high caseinase activity.

Supplier: STEMCELL Technologies

Minimum Essential Medium Eagle - Alpha modification (Alpha MEM) with nucleosides.

Supplier: MP Biomedicals

L-Lysine is an essential proteinogenic α amino acid. It contains an N-butyl amino group in the side chain, and this moiety is protonated at physiological pH. In addition, L-lysine is one of the two amino acids that are degraded to give ketone bodies (a ketogenic amino acid).

Supplier: MP Biomedicals

Storage: Store at room temperature (15-30 °C)
L-Tyrosine is one of the three aromatic amino acids, and is formed from the hydroxylation of phenylalanine.
L-Tyrosine is used in cell culture media and is a component of MEM amino acids solution. L-Tyrosine has been used in a cell culture study of the amino acid transport system b0,+ in epithelial cells isolated from chicken jejunum.

Supplier: MP Biomedicals

L-Glutamine, the uncharged and amidated analog of L-glutamic acid, is an important amino acid for the incorporation of NH4+ into biomolecules. It is biosynthesized from NH4+ and glutamate via the enzyme glutamate synthetase. In turn, degradation of glutamine to free the ammonia moiety is mediated by glutaminase. Glutamine also participates in acid-base regulation in vivo.

Supplier: US Biological

Dulbecco’s MEM is the most widely used modification of BME. It contains a four-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000 mg/ml glucose.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C). Store Desiccated.
L-Asparagine is used in cell culture media and is a component of MEM non-essential amino acids solution. L-Asparagine has been shown to enhance ornithine decarboxylase activity in cultured human colon adenocarcinoma Caco-2 cells and in cultured IEC-6 intestinal epithelial cells. Spore germination in Bacillus subtilis has been increased in the presence of L-asparagine. An isoxazoline RGD mimic platelet GPIIb/IIIa antagonist has been prepared by chiral synthesis with L-asparagine as a starting material. L-Asparagine has been utilized in the synthesis of 4-azalysine building blocks for application to combinatorial chemistry.

Supplier: MP Biomedicals

AlbumiNZ™ Bovine albumin is a single polypeptide chain consisting of approximately 583 to 595 amino acid residues and no carbohydrates. At pH 5 to 7 it contains 17 intrachain disulphide bridges and 1 sulphydryl group.

Supplier: MP Biomedicals

Minimum Essential Medium Eagle With Earle's Salts and 2,0 g/L sodium bicarbonate Without L-glutamine, L-cysteine, L-cystine, and L-methionine is generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Supplier: Corning

Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.

Supplier: MP Biomedicals

Storage: +4 °C
D-Biotin is a growth factor present in small amounts in every living cell. It is involved in naturally occurring carboxylation reactions. It occurs mainly bound to proteins or polypeptides. It is more abundant in the liver, kidney, pancreas, yeast and milk. Biotin levels are higher in cancerous tumors than in normal tissues. It is inactivated by binding to avidin.
D-Biotin may be used to elute proteins from avidin/streptavidin resins. It is widely used for dietary supplements and fortified foods. It is also used for tablets and hard-shell capsule preparation due to its pharmaceutical properties.
Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.
Soluble in water (22 mg/100 mL), ethanol (80 mg/100 mL), more soluble in hot water and in dilute alkalies; insoluble in other common organic solvents. Soluble in 2 M Ammonium hydroxide (50 mg/mL - clear, colorless solution), dimethylformamide (1.7 mg/mL).

Supplier: Biorbyt

Cellastim is a recombinant human serum albumin (HSA) that has been produced from an animal-free expression system.

Supplier: MP Biomedicals

6-Benzylaminopurine is a plant cell division factor (a plant growth factor).

Supplier: MP Biomedicals

L-Proline is an amino acid. The conjugation of L-proline with imidazolium-based ionic liquid (IL)-modified magnetic nanoparticles forms a catalyst that can be magnetically recovered and employed in direct asymmetric aldol reaction. The solubility and stability of ezetimibe (EZT) has been reported to enhance on cocrystallization with L-proline.

L-Proline has been used:

Supplier: MP Biomedicals

L-Glutamic Acid is a non-essential amino acid for human development; referred to as an excitatory amino acid (EAA) due to its role in neurotransmission. Acts as a neurotransmitter at fast synapses; agonist at kainate, NMDA and quisqualate receptors. L-Glutamic acid is one of the two amino acids that contains a carboxylic acid group in its side chains.

Supplier: MP Biomedicals

Albumins are a group of acidic proteins which occur in the body fluids and tissues of mammals and in some plant seeds. Serum and plasma albumin is carbohydrate-free and comprises 55 to 62% of the protein present. However, only about 40% of the total albumin in the body is in the circulating plasma at one time with the remainder being in extracellular spaces with which there is, in general, equilibration about every 24 hours.