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cDNA Synthesis Kit

cDNA Synthesis Kit

cDNA synthesis kit for miRNA, 50
Catalog # ORIGHP100042
New Product
Supplier:   OriGene
cDNA Synthesis Kit
Catalog # ORIGHP100042
Supplier:  OriGene
New Product
CAS Number:  
cDNA Synthesis Kit

cDNA synthesis kit for miRNA, 50

Specifications
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Product Details & Documents

qSTAR first-strand cDNA synthesis kit for miRNA is a two-step protocol kit.

The miRNA cDNA then becomes an effective qPCR template in SYBR Green I based qPCR method. The kit is uniquely designed to function only with OriGene’s miRNA detection system.

Delivery information: Kit includes 1 Vial poly (A) polymerase reaction 10X buffer [55 µl], 1 Vial poly (A) polymerase (5U/ μl) [55 µl], 1 Vial 10 mM ATP [55 µl], 1 Vial 5X MMLV buffer (dNTPs included)[350 µl], 1 Vial MMLV Reverse Transcriptase (200 U/µl)[55 µl], 1 Vial Oligo dT mix [55 µl], 1 Vial control primer pair (U6) [500 µl].

Specifications

Specifications

  • Description:
    cDNA synthesis kit for miRNA
  • No. of reactions:
    50
  • Cat. No.:
    ORIGHP100042
  • Storage conditions:
    Should be stored at –20 °C

Specifications

   
Acetonitrile, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

  #: 83639.320

Supplier: VWR Chemicals

Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

  #: 20864.320

Supplier: VWR Chemicals

VWR®, Transfer Pipettes, High Performance

  #: 612-1681

Supplier: VWR Collection

Acetone ≥99%, TECHNICAL

  #: 20063.365

Supplier: VWR Chemicals

VWR® Serological Pipette, Standard Line

  #: 612-3700

Supplier: VWR Collection

VWR®, Nitrile Gloves

  #: 112-3101

Supplier: VWR Collection

Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

  #: 83640.320

Supplier: VWR Chemicals

VWR® Facial Tissues

  #: 115-0600

Supplier: VWR Collection

Nitrile gloves, Kimtech™ Purple Nitrile™

  #: 112-4847

Supplier: Ansell

Acetonitrile ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®

  #: 1.00030.2500

Supplier: Merck

pH indicator strips, MQuant®, Supelco®

  #: 1.09535.0001

Supplier: Merck

Sealing film for manual application, Parafilm® M

  #: 291-0057

Supplier: HEATHROW

VWR® NITRILE LIGHT, Nitrile Gloves

  #: 112-2754

Supplier: VWR Collection

Water, HiPerSolv CHROMANORM® for HPLC

  #: 23595.328

Supplier: VWR Chemicals

Sodium hydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution

  #: 31770.294

Supplier: VWR Chemicals

VWR® AVS TITRINORM Buffer Solutions, 20 °C

  #: 32096.291

Supplier: VWR Chemicals

Water, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

  #: 83645.320

Supplier: VWR Chemicals

Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®

  #: 1.09439.1000

Supplier: Merck

VWR® Nitrile Extra Light, Nitrile Gloves

  #: 112-4195

Supplier: VWR Collection

epT.I.P.S.® Bulk - Pipette Tips

  #: 613-3508

Supplier: EPPENDORF