Specifications

Description:
EasySep™ human memory B cell isolation kit, 2×10⁹ cells
Pk:
1 KIT

Specifications

About this item

Immunomagnetic positive selection of human memory B cells.

Highly purified human memory B cells isolated in as little as 33 minutes
No-wash removal of EasySep™ Releasable RapidSpheres™
Optional isolation of untouched naïve B cells from the same sample

The EasySep™ Human Memory B Cell Isolation Kit is a two-step method designed to isolate CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells. First, CD27+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD27+ memory B cells that are immediately ready for downstream applications. An optional protocol allows for the isolation of naïve B cells in parallel for use in functional studies.

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