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Human Factor VII ELISA Kit
  ANTIA79314-96
 :  ANTIBODIES.COM
New Product
 :  
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Human Factor VII ELISA Kit
  ANTIA79314-96
 :  ANTIBODIES.COM
New Product
 :  

 

  • Pk:
    96 Tests
  • Assay duration:
    Multiple steps
  • Assay Type:
    Sandwich
  • Format:
    Pre-coated
  • Primary antibody reactivity:
    Human
  • Target protein:
    Factor VII
  • Description:
    Human Factor VII ELISA kit
  • Sample type:
    Serum, plasma, tissue homogenates and other biological fluids
  • Cross reactivity:
    Human Factor VII ELISA Kit exhibits high specificity and excellent specificity for the detection of human Factor VII. No significant cross-reactivity or interference between Factor VII and analogues was observed.
  • Detection method:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Assay Principle:
    Quantitative
  • Shelf life:
    Store for 6 months at 4 °C
  • Detection range:
    31,25 - 2000 pg/ml
  • Storage temperature:
    4 °C
  • Sample volume:
    100 μl
  • Sensitivity:
    18,75 pg/ml
  • Regulatory status:
    RUO

 

 

Human Factor VII ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Factor VII in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready To Use ELISA Kit
  • Detection Range: 31,25 to 2000 pg/ml
  • Sensitivity: 18,75 pg/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%

Human Factor VII ELISA Kit (A79314) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Factor VII in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Factor VII has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Factor VII present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Factor VII Antibody, which binds the captured Factor VII present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Factor VII captured in each well. The concentration of Factor VII can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

 : This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.