Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:Pancreatic Polypeptide
- Description:Mouse Pancreatic Polypeptide ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Mouse Pancreatic Polypeptide ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse Pancreatic Polypeptide. No significant cross-reactivity or interference between Pancreatic Polypeptide and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:31,25 - 2000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:18,75 pg/ml
- Regulatory status:RUO
Specifications
About this item
Mouse Pancreatic Polypeptide ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Pancreatic Polypeptide in serum, plasma, tissue homogenates, and other biological fluids.
- Ready To Use ELISA Kit
- Detection Range: 31,25 to 2000 pg/ml
- Sensitivity: 18,75 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra - Assay: CV <8%, Inter - Assay: CV <10%
Mouse Pancreatic Polypeptide ELISA Kit (A79655) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Pancreatic Polypeptide in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Pancreatic Polypeptide has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Pancreatic Polypeptide present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Pancreatic Polypeptide Antibody, which binds the captured Pancreatic Polypeptide present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Pancreatic Polypeptide captured in each well. The concentration of Pancreatic Polypeptide can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.