Stimulating T cells with BKV (LT) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. BKV Peptide pool is a lyophilised mixture of 171 peptides from the Large T (LT) antigen of BK polyomavirus (BKV), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 695 on LT antigen. LT antigen is an early viral protein (Low et al.) that contributes to the transformation and maintenance of host cells (Nakshatri et al.). It has been found to bind to a subset of tumor suppressor proteins (Harris et al.), and its co-expression with p53 has been observed in polyomavirus-associated nephropathy (Seemayer et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.