Kit including BrainPhys™ Neuronal Medium, SM1 Neuronal Supplement, and N2 Supplement-A for culture of ES/iPS cell-derived neurons.

Culture, differentiate, and mature neurons derived from human embryonic stem (ES) or induced pluripotent stem (iPS) cells in a complete medium optimised to promote, rather than inhibit neuronal activity. For your convenience, BrainPhys™ Neuronal Medium N2-A & SM1 Kit includes BrainPhys™ Neuronal Medium (basal medium) and supplements for culturing human ES/iPS-derived neuronal progenitor cells. Based on the formulation by Bardy and Gage (Bardy et al. PNAS, 2015), serum-free BrainPhys™ Neuronal Medium mimics the extracellular environment of the central nervous system (CNS) to yield a higher proportion of synaptically active neurons.

Brewer’s B27-based NeuroCult™ SM1 Neuronal Supplement ensures cell health and encourages neurite outgrowth and branching in short- and long-term serum-free cultures, and N2 Supplement-A supports the in vitro differentiation of ES/iPS-derived cells to neuronal subtypes. For lineage-specific differentiation requiring growth factors, please see BrainPhys™ hPSC Neuron Kit, which also includes BDNF and GDNF. To avoid shocking cells with media changes, also use BrainPhys™ medium when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging. View our additional resources to learn more about the BrainPhys™system.