Serum-free neurophysiological basal medium for improved neuronal function.

Promote, rather than inhibit, neuronal activity and maturity in cultured primary or human pluripotent stem cell (hPSC)-derived neurons. Based on the formulation by Bardy and Gage (Bardy et al. PNAS, 2015), BrainPhys™ Neuronal Medium is a serum-free basal medium that is optimised to yield a higher proportion of synaptically active neurons by mimicking the central nervous system (CNS) extracellular environment. Use BrainPhys™ Neuronal Medium for long-term culture of hPSC- and CNS-derived neurons. To avoid shocking cells with media changes, also use BrainPhys™ medium when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging.

To ensure cell health in long-term serum-free culture, BrainPhys™ Neuronal Medium must be combined with an appropriate serum-replacement supplement, such as NeuroCult™ SM1 Neuronal Supplement and/or N2 Supplement-A. For your convenience, various BrainPhys™ kits that include the required supplement(s) for primary or hPSC-derived neurons are also available. View our additional resources to learn more about the BrainPhys™ system.