Media and supplements for differentiation of human pluripotent stem cells into blood vessels organoids.

Generate physiologically relevant blood vessel organoids - easily and with high yield - for tissue repair and regeneration studies or to model vascular disease in vitro by using STEMdiff™ Blood Vessel Organoid Kit. Use STEMdiff™ Blood Vessel Organoid Kit for efficient and reproducible generation of blood vessel organoids from human pluripotent stem cells (hPSCs), with the option to scale up for high-throughput screening in a 96-well format. Compared to homemade organoid culture media formulations, organoids generated with this easy-to-use kit develop extensive and physiologically relevant vascular networks and strong endothelial cell-pericyte interactions.

Blood vessel organoids generated using this kit have CD31+/CD34+/CD144+/KDR+ endothelial cells and PDGFR-β+/CD146+/SMA+/NG-2+ pericytes.The simple protocol also eliminates the need for serum screening and labor-intensive protocol optimisation. Use STEMdiff™ Blood Vessel Organoid Kit with mTeSR™ Plus or mTeSR™1 culture media for an easy transition from hPSCs to organoids. For convenience, STEMdiff™ Blood Vessel Organoid Maturation Medium, required for the maturation of hPSC-derived blood vessel organoids, is available either as a part of the kit or for individual sale. To differentiate hPSCs to endothelial cells, use our STEMdiff™ Endothelial Differentiation Kit.