For differentiation of human ES and iPS cells to monocytes.

Differentiate human pluripotent stem cells (hPSCs) to monocytes expressing CD14 with the feeder-free and serum-free STEMdiff™ Monocyte Kit. The simple protocol is performed in 2D adherent cultures. During the first 3 days, Medium A induces cells toward mesoderm. For the subsequent 4 days, mesodermal cells are further differentiated toward the hematopoietic lineage using Medium B. At Day 7, the medium is changed to Monocyte Differentiation Medium, which facilitates the differentiation to monocytes. CD14+ monocytes can be harvested directly from the culture supernatant starting as early as Day 14 and can be repeatedly harvested during the rest of the culture period. Peak CD14+ frequency is typically 60 to 80%.

The hPSC-derived monocytes can be further differentiated to dendritic cells or macrophages using the ImmunoCult™ Dendritic Cell Culture Kit or the ImmunoCult™-SF Macrophage Medium, respectively. For your convenience, components required for the preparation of the Monocyte Differentiation Medium, StemSpan™ SFEM II and STEMdiff™ Monocyte Differentiation Supplement (100X), are also available for purchase individually.