Cell culture medium for establishment and maintenance of mouse hepatic progenitor organoids.

HepatiCult™ Organoid Growth Medium (Mouse) is a serum-free, defined cell culture medium for establishment and maintenance of mouse hepatic progenitor organoids. These organoids, or 'mini-livers', provide an in vitro organotypic culture system for studying hepatic stem and progenitor cells. Organoids grown in HepatiCult™ feature an epithelium expressing genes marking hepatic stem and progenitor cells (PROM1, AXIN2, SOX9 and CD44), ducts (KRT19 and HNF1b) and hepatocytes (HNF4a, AFP).

Hepatic organoids can be passaged every 4 to 7 days, can be cryopreserved, and are primed for downstream differentiation. HepatiCult™ supports mouse hepatic organoid culture either embedded in Corning® Matrigel® domes or in a dilute Matrigel® suspension. Organoid culture enables convenient in vitro characterization of the hepatic epithelium in a physiologically relevant system and reduces the need for animal use.