Animal component-free suspension culture medium for human ES and iPS cells.

TeSR™-E8™ 3D is an animal component-free culture medium developed for the expansion and scale-up of undifferentiated human embryonic stem (ES) and induced pluripotent stem (iPS) cells as aggregates in 3D suspension culture. Based on TeSR™-E8™, TeSR™-E8™3D is a low-protein medium with an optimised protocol for scaling up human ES and iPS cell production in suspension culture. The novel fed-batch workflow saves time and media; daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days. TeSR™-E8™3D is made with the highest level of quality and care. It contains only the essential components required for scale-up of ES and iPS cells. The TeSR™-E8™3D system is compatible with cells previously cultured in mTeSR™1 or TeSR™-E8™ on Corning® Matrigel® hESC-qualified matrix or Vitronectin XF™, a matrix developed and manufactured by nucleus biologics), and is compatible with a range of suspension culture vessels.