The Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic chromatography utilising a hydrophobic enzyme. The use of the enzyme allows purification followed by a simple enzyme assay to detect the fractions that contain the enzyme. This lab activity involves preparation of a crude protein extract and running hydrophobic chromatography to isolate the enzyme.

• The principle of hydrophobic chromatography
• Immobilisation of protein on hydrophobic columns
• Factors influencing binding and elution of proteins on hydrophobic columns
• Hands-on hydrophobic chromatography lab activity

Also required: Low speed centrifuge for 1,5 to 2 ml tubes, water bath or beaker and thermometer.