The Affinity Chromatography kit teaches the basic principle of affinity chromatography utilising highly specific affinity columns. This lab activity involves preparation of a crude protein extract and running affinity exchange chromatography for isolation of a protein.

• The principle of affinity chromatography
• Immobilisation of protein on affinity columns
• Binding and elution of proteins on affinity columns
• Factors influencing affinity chromatography
• Hands-on affinity chromatography lab activity

Also required: Spectrophotometer and cuvettes or microplate reader and microplate (optional), low speed centrifuge for 1,5 to 2 ml tubes.