Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

• For 6 lab groups
• Requires 60 to 90 minutes

Also required: Spectrophotometer and cuvettes, ring stands and clamps, and 5 ml pipettes.