This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.

• For 10 Lab Groups
• Complete in 35 minutes followed by overnight incubation

Also required: Automatic micropipettes with tips, 5 or 10 ml pipettes, 55 °C water bath, measuring spatulas or toothpicks, microwave or hot plate, distilled water, and incubator (optional).