Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

• For 5 lab groups
• Complete in 60 minutes

Also required: Electrophoresis tank and power supply, water batch, microcentrifuge (10000 min⁻¹), 5 to 50 µl adjustable or 40 and 50 µl fixed volume micropipettes, 95 to 100% isopropanol, and white light box.