Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  Fc Tag
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Immobilised Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) on CM5 Chip can bind Human TACI/TNFRSF13B, Fc Tag (Cat. No. TAI-H5256) with an affinity constant of 0.658 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Immobilised Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human BAFFR, Llama IgG2b Fc Tag, low endotoxin (Cat. No. BAR-H5258) with an affinity constant of 44,5 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) on Protein A Biosensor, can bind Biotinylated Human BCMA, His,Avitag (Cat. No. BCA-H82E4) with an affinity constant of 0,69 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) stimulates proliferation of mouse spleen cells. The EC50 for this effect is 4,91 - 9,81 ng/ml (Routinely tested).
• Gene ID:
  Q9Y275-1
• Protein synonyms:
  CD257|ZTNF4|THANK|TALL-1|TNFSF20|DTL|TNFSF13B|TALL1|BLYS|BAFF
• Protein/peptide name:
  BAFF
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular weight:
  76,7 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before Lyophilisation.
• Shipping temperature:
  RT