ROS Detection Assay Kit is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2', 7'-Dichlorodihydrofluorescein diacetate (DCF-DA). Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidised by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.

• Measure ROS production in live cells
• Ideal for fluorescence microscopy, microplate titration and cytometry
• Compatible with adherent cells as well as suspension cells

Upon receipt, store the DFC-DA tube at –20 °C. Avoid multiple freeze/thawing cycles. Store all other components at 4 °C.