Specifications
- Antibody type:Primary
- Antigen name:Topoisomerase 2 alpha
- Antigen symbol:TOP2A
- Clonality:Monoclonal
- Clone:1C5
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoPrecipitation:Yes
- Isotype:IgG2a
- Reactivity:
- Western blot:Yes
- Seq:02
- Environmentally Preferable:
- Epitope:C-Terminal
- Form:Liquid
- Antigen synonyms:alpha isozyme|DNA topoisomerase 2-alpha|DNA gyrase|DNA topoisomerase (ATP-hydrolyzing)|TP2A|170 kD|DNA topoisomerase II|TOP2
- Storage buffer:PBS, pH 7,2, containing 50% glycerol
- Storage temperature:–20 °C
- Shipping temperature:Shipped on blue ice
- Immunogen:Recombinant human DNA-topoisomerase IIalpha (C-terminal fragment)
- Purification:Purified by Protein A affinity chromatography
- Size:100 µg
- Pk:100 µG
Specifications
About this item
DNA-topoisomerase II, also known as Topo II or Top 2, is a multifunctional nuclear enzyme required during DNA replication, chromosome disjunction at mitosis, and other DNA-related activities because of its ability to alter DNA supercoiling. In mammalian cells, Topo II consists of two isozymes, Topo II-alpha (170kDa) and Topo II-beta (180kDa) and expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo II-beta is expressed constitutively throughout the cell cycle, whereas the expression of Topo II-alpha is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. DNA-topoisomerase II is the target of some of the most active drugs used in chemotherapy and has been shown to have a critical role in neural development.
Type: Primary
Antigen: TOP2A
Clonality: Monoclonal
Clone: 1C5
Conjugation: Unconjugated
Epitope: C-Terminal
Host: Mouse
Isotype: IgG2a
Reactivity: Human