E. coli

Bovine

Lyophilized Leptin although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution Leptin should be stored at 4 °C between 2-7 days and for future use below −18 °C. For long term storage it is recommended to add carrier protein (0. 1% HSA or BSA). Please prevent freeze/thaw cycles.

Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

LEP

Leptin

Greater than 95.0% as determined by RP-HPLC and analysis by SDS-PAGE

It is recommended to reconstitute the lyophilized Leptin in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.

The protein was lyophilized from concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

SDS-PAGE , HPLC , FuncS