Specifications

• Source:
  E. coli
• Species:
  Human
• Storage conditions:
  Store at 4 °C for up to two weeks. For long term storage, aliquot and store at −20 °C, avoid freeze/thaw cycles.
• Biological activity:
  The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is 0.1 ng/ml corresponding to specific activity of x 10,000,000 Units/mg.
• Protein synonyms:
  Urogastrone
• Protein/peptide name:
  EGF
• Purity:
  > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Solubility:
  It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence:
  NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.
• Formulation:
  The protein was lyophilized from concentrated (1mg/ml) solution containing 10mM Phosphate buffer pH-7.4.
• Tested applications:
  SDS-PAGE