The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.

• Time-resolved fluorescence detection
• Europium-labeled goat, anti-rabbit, anti-mouse or streptavidin labeled antibodies
• Supports nitrocellulose and PVDF membranes
• 340/80 nm excitation with 616/10 nm emission range
• Optimised for use with ScanLater™ Western Blot Detection Cartridge for the SpectraMax® i3 and Paradigm® Multi-Mode Platforms

Using this Western blot method, researchers can eliminate time-dependent substrate addition steps and sustain blot signal stability for at least one month. Researchers can also enhance assay sensitivity using TRF method to reduce background noise. This kit allows researchers to maintain femtogram to picogram protein sensitivity, similar to traditional western blot detectors.