Pierce™ HisPur™ Cobalt Purification Kit is designed for the efficient purification of recombinant polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells.

• Obtain <10 mg of pure His-tagged protein per ml of resin
• Resin can be reused several times or discarded after single use
• Cobalt-chelate coordination core binds fewer host protein contaminants
• Purify proteins under native or denaturing conditions

His-tagged proteins are purified from total soluble protein extract using a cobalt-charged tetradentate chelator immobilised onto 6% crosslinked agarose. Many immobilised metal affinity chromatography (IMAC) resins contain nickel (Ni²⁺) for purifying His-tagged proteins. Although Ni²⁺ chelate resins achieve high protein yields, purity is often sub-optimal, requiring additional clean-up steps. Cobalt achieves both high yield and purity with minimal optimisation. The HisPur™ Cobalt Resin binds fewer non specific proteins, displays less metal leaching and enables less stringent elution conditions compared to Ni²⁺ resins.