Classic IP kit, Pierce™
PIER26146
:
- Pk:1 KIT
- Description:Classic IP kit, sufficient for at least 50 IP reactions with 10 µl resin
The Pierce™ Classic IP Kit uses protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays.
- Spin columns eliminate resin loss and provide efficient separation of solutions
- Immunoprecipitate (IP) in less than one hour
- Recover antigen without harsh detergents or reducing agents
- Purify target protein from crude lysate in four simple steps
This kit involves formation of antibody:antigen complexes in a sample solution and then capture of that complex to an IgG-binding protein that is covalently bound to beaded agarose resin. The microcentrifuge spin column format helps to ensure effective washing and separation of samples from the beaded agarose affinity resin.
: The kit includes protein A/G plus agarose, 0,55 ml; IP lysis/wash buffer, 2×50 ml; conditioning buffer (100X), 5 ml; tris-buffered saline (20X), 25 ml; elution buffer, 50 ml; lane marker sample buffer (5X), 5 ml; control agarose resin, 2 ml; spin columns and collection tubes.