Pierce glutathione coated plates are coated with intact glutathione (GSH) and pre-blocked for use in capture and detection assays involving glutathione-S-transferase (GST) fusion proteins. These coated plates can be used in the analysis of cell lysates to determine the presence and concentration of the GST-fusion protein produced in an expression system. Plates can also be used to capture and coat fusion proteins via their GST tag for subsequent binding assays involving the recombinant protein. As the plates are pre-blocked, initial purification of the cell lysates is not necessary.

• Specifically binds fusion proteins containing glutathione S-transferase
• Detergents used to lyse cells do not inhibit GST protein binding to pre-coated plates (by contrast with direct coating methods with polystyrene plates)
• Ideal for analysis of cell lysates and to capture and coat fusion proteins via GST tag
• Pre-blocked with SuperBlock™ buffer to reduce non specific binding
• Binding capacity approimately 10 ng purified GST per well