Cronobacter sakazakii (formerly Enterobacter sakazakii) is found naturally in the environment and can survive in dry conditions. It occurs in dry foods, such as powdered infant formula, milk powder and starches as well as in teas. Especially young infants are at high risk developing severe meningitis, if infected. All common Real-Time PCR block cyclers are usable for the test. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons. The test can also be used for the confirmation of bacterial colonies. The Real-Time PCR Test allows the detection of the species with high accuracy and is more precise than, cultural methods often are.

• TaqMan probes
• Contains internal amplification control in HEX channel
• Taget in FAM channel
• Contains UNG to protect from re-amplification
• Positive DNA and negative control included