Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.

SHC1(SHC-transforming protein 1), also known as SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. By Southern analysis of somatic cell hybrids followed by both isotopic and fluorescence in situ hybridization, Huebner et al.(1994) assigned the SHC1 gene to 1q21. Yulug et al.(1995) used fluorescence in situ hybridization to map the SHC1 gene to 1q21. By the same method, an SHC-related sequence(SHCL1) was mapped to 17q21-q22. By FISH analysis and direct sequencing of vectorette library PCR products, Harun et al.(1997) identified SHC1P1, a 3.2-kb processed pseudogene, in Xq12-q13.1. SHC1P1 is 85% identical to mouse Shc p66. Reporter assays showed FKHRL1 transactivates CAT, suggesting a capacity to augment antioxidant scavenging. Nemoto and Finkel(2002) concluded that there is an important functional relationship between forkhead proteins(e.g., FKHRL1), SHC1, and intracellular oxidants, all of which are though to be involved in the aging process in worms and mammals.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

WB: The detection limit for SHC1 is approximately 1ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Type: Primary
Antigen: SHC
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: near the C-terminus
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat