Phusion® Green High-Fidelity DNA Polymerase
Phusion® Green High-Fidelity DNA Polymerase is a combination of Phusion® DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
- Highest fidelity
- Polymerase speed allows short extension times (15 to 30 s/kb)
- Robust performance, minimal optimisation needed
- Increased product yields with minimal enzyme amounts
- Direct loading on gel with Phusion® Green buffer
: Kit contains Phusion® DNA Polymerase (2 U/µl), 5X Phusion® Green HF Buffer, 5X Phusion® Green GC Buffer, DMSO and 50 mM MgCl₂. Both Phusion® and Phusion® Green buffers provide 1,5 mM MgCl₂ in the final 1X concentration.
Frequently Bought Together
Eppendorf Tubes® 3810X Microtubes 1,5 ml
: EPPENDORF
VWR®, Centrifuge Tubes, Conical Bottom
: VWR Collection
VWR® Serological Pipette, Standard Line
: VWR Collection
VWR®, Syringe Filters
: VWR Collection
Transfer pipettes
: RATIOLAB
VWR® Standard Line Transfer Pipettes
: VWR Collection
VWR®, Transfer Pipettes, High Performance
: VWR Collection
Safe-Lock Tubes, 1,5 ml - Microtubes
: EPPENDORF
VWR®, Spreaders, L-shaped, Disposable
: VWR Collection
Cell culture media, RPMI 1640
: BIOWEST
Multifunction rotators, PTR-35, PTR-60, 360°
: GRANT INSTRUMENTS
Phusion® High-Fidelity DNA Polymerase
: FINNZYMES REAGENTS