You searched for: Electrophoresis Reagents
Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
VWR®, Protein marker III (pre-stained), peqGOLD
Supplier: VWR Chemicals
Protein Marker III for monitoring protein separation, transfer efficiency and size determination.
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DNA ladder, O'RangeRuler™
Supplier: Thermo Fisher Scientific
O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.
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Lambda DNA phage, Hind III digest
Supplier: Rockland Immunochemicals
Lambda DNA fragments prepared by the digestion of purified lambda DNA with the restriction endonuclease Hind III.
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Lambda DNA/HindIII Marker
Supplier: Thermo Fisher Scientific
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
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Peqlab peqGOLD, Protein Marker IV (Pre-Stained)
Supplier: VWR Chemicals
peqGOLD pre-stained protein marker IV is a mixture of 10 recombinant, highly purified coloured proteins supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS-polyacrylamide gels.
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Peqlab peqGOLD, DNA Ladder, 100 bp Plus
Supplier: VWR Chemicals
peqGOLD DNA ladder 100 bp plus is ideal for size determination in the range of 100 to 3000 base pairs. There are no unspecific bands besides the fragments described. 1000 and 500 bp bands have a higher DNA content and serve as a size reference.
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VWR® RiboReady™, Color RNA Ladder, 1 kb
Supplier: VWR Chemicals
RiboReady™ Color 1 kb RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels.
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VWR®, DNA ladders
Supplier: VWR Chemicals
VWR® DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.
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VWR® PCR DNA Marker
Supplier: VWR Chemicals
PCR DNA Marker™ offers 8 fragments ranging from 50 to 2000 bp. The marker contains high intensity reference bands that are ideal for quick sizing of PCR products and restriction digests. Simply mix 10 μl of PCR DNA Marker™ with required quantity of loading buffer and load into well. There is sufficient material for 100 assays.
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Peqlab peqGOLD, DNA Ladder, 1 kb
Supplier: VWR Chemicals
DNA ladder for quantitative and qualitative analysis of dsDNA.
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VWR®, DNA ladder, 100 bp
Supplier: VWR Chemicals
Ladder with 13 fragments ranging from 100 to 3000 bp for easy band identification.
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VWR® EZ-Vision®, DNA Ladder, 100 bp
Supplier: VWR Chemicals
EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.
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Restore™ Western Blot Stripping Buffer
Supplier: Thermo Fisher Scientific
Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.
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Peqlab peqGOLD, Protein Marker II (Unstained)
Supplier: VWR Chemicals
Protein Marker II for accurate molecular weight determination of proteins by SDS-PAGE.
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VWR® RiboReady™ Color Micro RNA Ladder
Supplier: VWR Chemicals
RiboReady™ Color Micro RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels. The apparent sizes of bands in the RiboReady™ Color Micro RNA ladder are 100, 75, 50, 40, 30 and 20 bases in length.
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S-Nitrosylation western blot kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.
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Fluorescent western blot stripping buffer, Restore™
Supplier: Thermo Fisher Scientific
Restore™ Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.
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Western blot enhancer, SuperSignal™, Pierce
Supplier: Thermo Fisher Scientific
SuperSignal™ Western Blot Enhancer is a membrane treatment and a primary antibody diluent that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a level at which it could not be detected without enhancement.
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Destain solutions
Supplier: G-Biosciences
G-Biosciences' Destain I contains approximately 45% methanol and 9% glacial acetic acid. G-Biosciences' Destain II contains approximately 5% methanol and 7% acetic acid.
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Pierce™ Western Blot Signal Enhancer
Supplier: Thermo Fisher Scientific
Western blot signal enhancer is a membrane treatment that has been shown to increase intensity of a target protein band on a Western blot and to enable detection of a target analyte at a Level at which it could not be detected without enhancement.
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Fluorogenic peroxidase substrate kit, QuantaBlu™
Supplier: Thermo Fisher Scientific
QuantaBlu™ Fluorogenic peroxidase substrate kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.
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Pierce™ Biotin 3' End DNA Labelling Kit
Supplier: Thermo Fisher Scientific
This Biotin 3' End DNA Labelling Kit is used for tagging single-stranded DNA primers with biotin for use in non radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.
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Far-western blot kit for biotinylated proteins, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins through interaction with any biotin-labeled test protein used as the primary probe. On-membrane detection offers greater sensitivity whereas in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer.
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Peqlab peqGOLD, DNA Ladder Mix
Supplier: VWR Chemicals
DNA ladder for quantitative and qualitative analysis of dsDNA.
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VWR®, DNA marker, 1 kb
Supplier: VWR Chemicals
DNA marker consists of 11 fragments ranging from 500 to 10000 bp for easy band identification.
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Chemiluminescent RNA EMSA kit, LightShift™
Supplier: Thermo Fisher Scientific
LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
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SuperSignal™ Pierce™ West Pico PLUS chemiluminescent substrate
Supplier: Thermo Fisher Scientific
SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by Western blot analysis.
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Pierce™ OPD (o-phenylenediamine Dihydrochloride)
Supplier: Thermo Fisher Scientific
OPD (o-phenylenediamine dihydrochloride) is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492 nm by ELISA plate readers.
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Western Blotting Substrate, Pierce™ ECL Plus
Supplier: Thermo Fisher Scientific
The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
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Western blotting kits, SuperSignal™ west pico chemiluminescent substrate kits, Pierce™
Supplier: Thermo Fisher Scientific
These kits include SuperSignal™ West Pico Substrate that is a highly sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. The kits also contain reagents for the specific detection of biotinylated antibodies, rabbit IgG or mouse IgG. The substrate’s intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.