You searched for: Nucleic Acid Reagents
The wide range of reagents are suitable for use with nucleic acids in transfection and transformation procedures, as well as cloning, sequencing, purification, and extraction. Use these reagents for isolating and purifying nucleic acids from biological material for use in further laboratory procedures, and their ready-to-use status makes them easy and straightforward to apply to nucleic acids upon opening. Nucleic acid reagents are tailored to suit a number of different and varied applications.
Fluorescent nucleotides, Red 594 dUTP and Red 598 dUTP
Supplier: ENZO LIFE SCIENCES
Red dUTP's (Red 594 dUTP, Red 598 dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
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VWR®, TEMPase Hot Start DNA polymerase
Supplier: VWR Chemicals
VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR. A glycerol-free TEMPase Hot Start DNA Polymerase is also available for automation and freeze drying.
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Brilliant II probe MM
Supplier: AGILENT
Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
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QuikChange II
Supplier: AGILENT
These QuikChange II kits provide improved fidelity, while maintaining greater than 80% mutation efficiency for single site mutagenesis.
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Universal reverse primer
Supplier: AGILENT
The universal reverse primer serves as the downstream primer in the qPCR reaction by annealing to a universal sequence tag that is added to the cDNA during the reverse transcription protocol.
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dNTP set
Supplier: Biotium
Highly purified dNTPs (>99% by HPLC) in purified water. Each deoxynucleotide solution (dATP, dCTP, dGTP, dTTP) is provided in a separate vial at 100 mm.
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TriLink® N1-Methyl-Pseudouridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
N1-Methyl-Pseudouridine-5'-Triphosphate is widely used in mRNA synthesis for therapeutic and vaccine development to improve mRNA functionality. This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.
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AMPIGENE® qPCR 1-Step Probe Kit No-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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dNTP mix
Supplier: Biotium
Highly purified mix of dNTPs (>99% by HPLC) in 0,6 mM Tris-HCl (pH 7,5); each dNTP is at a concentration of 10 or 25 mm in the solution provided.
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Paq5000 DNA polymerase
Supplier: AGILENT
Paq5000 is capable of quickly and robustly amplifying up to 6 kB genomic DNA targets.
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Bio-17-ATP
Supplier: ENZO LIFE SCIENCES
Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for in vitro transcription reaction catalysed by T3, T7 or SP6 RNA polymerases.
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Viability PCR starter kits
Supplier: Biotium
Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).
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TriLink® T7 RNA Polymerase
Supplier: TriLink BioTechnologies
T7 RNA Polymerase is a wild-type enzyme to synthesize RNA from DNA templates by in vitro transcription.
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AMPIGENE® HS Taq DNA Polymerase
Supplier: ENZO LIFE SCIENCES
Increased sensitivity standard PCR for a broader range of samples, with enhanced speed, yield, and specificity.
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AMPIGENE® qPCR 1-Step Green Kit Lo-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and dye-based qPCR in a single tube.
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TriLink® CleanCap® Reagent AG (3'OMe)
Supplier: TriLink BioTechnologies
TriLink's patented CleanCap® Reagent AG (3' OMe) is a co-transcriptional capping reagent for in vitro transcription of 5' capped mRNA resulting in a Cap 1 structure with >95% capping efficiency.
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DpnI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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TriLink® CleanCap® Reagent AG
Supplier: TriLink BioTechnologies
CleanCap® Reagent AG is TriLink’s patented co-transcriptional capping reagent for in vitro transcription of 5’ capped mRNA resulting in a Cap 1 structure. CleanCap has shown to provide up to 98% capping efficiency.
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Qscript lyo 1-step
Supplier: Quantabio
Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.
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Brilliant II SYBR® master mix with ROX
Supplier: AGILENT
Brilliant II SYBR® QPCR and QRT-PCR master mix kits with high/low Rox offer superior sensitivity for improved quantification and reproducibility.
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Brilliant II SYBR® Master mix
Supplier: AGILENT
Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
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TriLink® 2'-Fluoro-2'-deoxycytidine-5'-Triphosphate
Supplier: TriLink BioTechnologies
2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability.
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AMPIGENE® Taq mix
Supplier: ENZO LIFE SCIENCES
Optimised standard PCR mix for enhanced speed, yield, and specificity. Used for standard PCR applications.
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miRNA QRT-PCR detection reagent
Supplier: AGILENT
Polyadenylation of miRNA from total RNA and synthesis of first-strand cDNA from tailed miRNAs.
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Restriction enzymes, NotI, 50 U/µl, Fermentas
Supplier: Thermo Fisher Scientific
GGCCGC sites and cuts best at 37 °C in O buffer.
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Site-directed mutagenesis kits, QuikChange Lightning
Supplier: AGILENT
The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours.
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Restriction enzymes, HindIII, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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TriLink® 5-Propargylamino-2'-deoxyuridine-5'-Triphosphate
Supplier: TriLink BioTechnologies
5-Propargylamino-2'-deoxyuridine can be enzymatically incorporated into DNA and cDNA to introduce primary amine groups. Primary amines can be labeled with NHS ester fluors and haptens for detection and immobilisation of the resulting DNA strand.
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Master mixes, brilliant HRM ultra-fast loci
Supplier: AGILENT
The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.
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MycoSensor qPCR assay kits
Supplier: AGILENT
The MycoSensor PCR and qPCR assay kits detect cultured Mycoplasma infection by real-time qPCR or PCR.