You searched for: Nucleic Acid Reagents

Supplier: Oxford Nanopore Technologies

Additional flow cell priming reagents to enable additional loading of more library during a sequencing run.

Supplier: MP Biomedicals

Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.

Supplier: Oxford Nanopore Technologies

A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples.  Contains all Oxford Nanopore sequencing reagents for rapid whole-genome sequencing of SARS-CoV-2.

Supplier: Quantabio

Extracta™ DBS is a ready to use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman® 903 filter/TFN or 226 from Ahlstrom Munksjö paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix® or PerfeCTa MultiPlex qPCR ToughMix® enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

Supplier: MP Biomedicals

The GeneClean® Kit is designed to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels.

Supplier: MP Biomedicals

FastRNA® Pro Blue Kit is used to isolate total RNA from gram positive and gram negative bacteria. Isolated RNA is suitable for RT-PCR analysis and other downstream applications.

Supplier: MP Biomedicals

FastRNA™ Pro Soil-Direct Kit is used to isolate total RNA from organic material found in soil samples. The total RNA isolated is suitable for RT-PCR analysis and other downstream applications.

Supplier: MP Biomedicals

The GeneClean® Spin Kit is used to purify fragments of DNA from 200 bp to 300 kb from solutions and agarose gels. The kit includes spin filters whose usage prevents silica particle carry-over into cleaned DNA.

Supplier: OMEGA BIO-TEK

Mag-Bind® SeqDTR™ is designed for the effective and reliable removal of unincorporated terminators from sequencing reaction. Sequencing products are first mixed with the Mag-Bind® SeqDTR™. DNA then selectively binds to the Mag-Bind® SeqDTR™ particles. With two rapid wash steps, trace contaminants such as nucleotides, primers and small, non targeted amplification products are removed.

Supplier: OMEGA BIO-TEK

Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent to sample ratio used, giving the user flexibility to perform left, right or double-sided size selection.

Supplier: Oxford Nanopore Technologies

Short Fragment Buffer (SFB).

Supplier: Quantabio

The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.

Supplier: Oxford Nanopore Technologies

Additional Long Fragment Buffer (LFB).

Supplier: Oxford Nanopore Technologies

The Rapid PCR Barcoding Kit 24 V14 provides a fast and simple method of preparing up to 24 barcoded samples for sequencing 1-5 ng of gDNA using PCR.

Supplier: Sartorius

The Vivapure® AdenoPACK™ adenovirus purification and concentration kits offer researchers who need to recover up to 3×10¹³ purified recombinant adenovirus particles for in vitro transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures.

Supplier: MP Biomedicals

MPure™ Viral Nucleic Acids Extraction Kit is used with the MPure-12 instrument for extraction of Viral DNA or RNA from human biological specimens such as serum, plasma, and other cell-free fluids.

Supplier: MP Biomedicals

The RNaid® Kit is designed to isolate and purify RNA (75 bases to 10 kb) from native and denaturing agarose and polyacrylamide gels.

Supplier: MP Biomedicals

The SPINeasy® RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.

Supplier: MP Biomedicals

FastRNA™ Pro Soil-Indirect Kit is used to isolate total RNA from organic material found in soil samples. This kit allows for the sample to be slurried with water or buffer in order to further separate biomass from the soil or environmental matrix, and the resulting supernatant is then filtered, lysed, and purified through the rest of the protocol. Total RNA isolated is suitable for RT-PCR analysis and other downstream applications.

Supplier: MP Biomedicals

The EZ-Yeast™ Transformation Kit is designed for high throughput transformation with efficiencies up to 10³ transformants per μg plasmid DNA. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. Perfect for a quick analysis of potentially positive two-hybrid colonies, it does not require fully competent cells prior to transformation.

Supplier: Quantabio

sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

Supplier: Oxford Nanopore Technologies

The cDNA-PCR Sequencing Kits enable the identification and quantification of full-length transcripts with highest output using nanopore sequencing.

Supplier: Oxford Nanopore Technologies

PCR Barcoding Expansion packs enable multiple nanopore sequencing libraries to be pooled and run on a single MinION Flow Cell.

Supplier: Oxford Nanopore Technologies

The 16S Barcoding Kit delivers rapid, genus-level bacteria identification using nanopore sequencing.

Supplier: Thermo Fisher Scientific

dNTP mix contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 2 mM.

Supplier: AAT BIOQUEST

10 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water.

Supplier: AAT BIOQUEST

This iFluor® 647-PEG12-dUTP conjugate has the same spectral properties as ChromaTide™ Alexa Fluor™ 647-dUTP.

Supplier: AAT BIOQUEST

Fluorescein-12-dCTP might be incorporated into a DNA sequence as its natural counterpart dCTP to incorporate a fluorescein tag in a DNA molecule.

Supplier: AAT BIOQUEST

3'-O-Azidomethyl-dGTP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).

Supplier: TriLink BioTechnologies

Inorganic Pyrophosphatase (iPPase) is an enzyme to remove pyrophosphate in IVT to drive the reaction forward.