You searched for: Electrophoresis Buffers
The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.
Protein blocking agent, BLOT-QuickBlocker™
Supplier: G-Biosciences
BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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G-Biosciences Sodium Chloride-Sodium Phosphate-EDTA Buffers (20X)
Supplier: G-Biosciences
Saline-sodium phosphate-EDTA hybridisation buffer [20X].
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Non-animal protein blocking Agent, NAP-BLOCKER™
Supplier: G-Biosciences
NAP-BLOCKER™ is an animal-free protein blocking agent recommended for improved assay sensitivity, minimal non specific binding and a high signal-to-background ratio. It is suitable for Western blots, dot blots and assay development.
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IHC Wash Buffer, Enzo Life Sciences
Supplier: Enzo Life Sciences
The IHC wash buffer is a for washing and removing excess reagents during immunostaining.
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VWR® Buffer, MES-SDS, 20X, UltraPure Grade
Supplier: VWR Chemicals
Useful for high resolution of proteins on neutral pH SDS-PAGE gels.
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SDS-Page running gel buffers, 10X concentrate
Supplier: Rockland Immunochemicals
SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.
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6X GelRed prestain loading buffer with tracking dye
Supplier: Biotium
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
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PBS tablets
Supplier: PanReac AppliChem
Tablets for preparation of 100 ml phosphate buffered saline (PBS).
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In-situ hybridisation buffer (1.25X concentrate), Enzo Life Sciences
Supplier: Enzo Life Sciences
In Situ Hybridization Buffer provides a high quality hybridization medium for in situ hybridization analyses using either DNA or oligonucleotide probes labeled with biotin-, digoxigenin- or fluorescein-modified nucleotides.
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Tween-Tris-buffered saline (TTBS), 10X concentrate, pH 7,5
Supplier: Rockland Immunochemicals
Tween Tris buffered saline is suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA.
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TAE buffer, 50X
Supplier: EDVOTEK
This 50X concentrated solution (Tris-acetate, EDTA, pH 7,8) is sufficient for making 5 L of diluted working buffer for making agarose gels and electrophoresis chamber buffer.
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Immunoassay Blocking (BSA Free)
Supplier: Abcam
Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.
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In-situ hybridisation buffer for HPV probes
Supplier: Enzo Life Sciences
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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G-Biosciences Hybridization Buffer (Hybridization Denaturing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)
Supplier: G-Biosciences
Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.
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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)
Supplier: Biotium
Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)
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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)
Supplier: Rockland Immunochemicals
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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G-Biosciences Hybridization Buffer (Hybridization Neutralizing Solution)
Supplier: G-Biosciences
Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.
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TAE buffer (20X), Pierce™
Supplier: Thermo Fisher Scientific
20X TAE buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Acetate EDTA running buffer used for agarose gel electrophoresis of nucleic acids.
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer) pH 8,3
Supplier: PanReac AppliChem
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer) pH 8,3
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Wash buffers, femto TBST™ and femto PBST™
Supplier: G-Biosciences
The femto TBST™ and femto PBST™ wash buffers will enhance sensitivity of immunoassays by minimising the 'washing out' of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds, resulting in a larger ratio of signal to background. These can be used for incubation and washing procedures.
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Pierce™ Blocking Buffers, Protein Free
Supplier: Thermo Fisher Scientific
These protein-free blocking buffers are either a PBS or a TBS formulation of a non protein compound that provides effective blocking for membrane based and plate based protein detection methods, resulting in extremely low background.
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Gel loading solution, 10X
Supplier: EDVOTEK
1 ml concentrate for 200 samples.
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VHB DNA Washing Buffer
Supplier: OMEGA BIO-TEK
VHB buffer must be diluted with ethanol before use.
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Wash buffer, Pierce™ Fast Wash Buffer
Supplier: Thermo Fisher Scientific
Pierce™ Fast Wash buffer, 10X, for use with Pierce™ Fast Blocking buffer.
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Buffer, TBE (Tris-borate-EDTA) powder for 10X concentrated solution
Supplier: PanReac AppliChem
Powder for 10X concentrated aqueous solution (170,28 g/L). Composed of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂.