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You searched for: Protein Assays

Protein Assays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Amplite® Fluorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® Fluorimetric Biotin Quantitation Kit uses Biotinylite™ Green, a fluorogenic biotin sensor.

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ReadiUse™ TCA Deproteinization Sample Preparation Kit

ReadiUse™ TCA Deproteinization Sample Preparation Kit

Supplier: AAT BIOQUEST

The analysis of amino acids, ions, metabolites and other small molecules is frequently hindered by the interference of lipids, protein and enzymes present in biofluids, cell and tissue lysates.

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Firefly luciferase flash assay kit, Pierce™

Firefly luciferase flash assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

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ReadiUse™ TCEP Removal Solution

ReadiUse™ TCEP Removal Solution

Supplier: AAT BIOQUEST

TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins.

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Surface protein plus test swabs, PRO100, Clean-Trace™

Surface protein plus test swabs, PRO100, Clean-Trace™

Supplier: 3M Food Safety

3M™ Clean-Trace™ Surface Protein Plus Test Swab PRO100 is a simple, quick, and reliable method of checking for the removal of protein residues after cleaning and quickly identifies contamination.

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Tetracysteine FLASH Reagent *2 mM DMSO Solution*

Tetracysteine FLASH Reagent *2 mM DMSO Solution*

Supplier: AAT BIOQUEST

FLASH is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other.

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Surface protein (allergen) test swab ALLTEC60, Clean-Trace™

Surface protein (allergen) test swab ALLTEC60, Clean-Trace™

Supplier: 3M Food Safety

3M™ Clean-Trace™ Surface Protein (Allergen) Test Swab ALLTEC60 is a rapid, simple, and reliable solution to monitor the presence of allergens on surfaces to effectively manage cleaning.

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Tetracysteine FLASH Diacetate *2 mM DMSO Solution*

Tetracysteine FLASH Diacetate *2 mM DMSO Solution*

Supplier: AAT BIOQUEST

FLASH diacetate is a cell-permeable version of FlASH, which is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other.

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Tetracysteine REASH Acetate *2 mM DMSO Solution*

Tetracysteine REASH Acetate *2 mM DMSO Solution*

Supplier: AAT BIOQUEST

REASH acetate is a cell-permeable version of REASH, which is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.

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Amplite® Rapid Colorimetric Biotin Quantitation Kit

Supplier: AAT BIOQUEST

Optimised to use with Nanodrop. Amplite™ colorimetric biotin quantitation kit is designed to quantify the number of biotins attached to a protein or other macromolecule with a nanodrop spectrometer.

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Solid phase biotinylation kits, EZ-Link™, Pierce™

Solid phase biotinylation kits, EZ-Link™, Pierce™

Supplier: Thermo Fisher Scientific

This antibody labeling system uses nickel chelated agarose to temporarily immobilise antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.

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Antibody labelling kits, HisTag® Antibody HRP Conjugate kit

Supplier: Merck Millipore (Novagen)

The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

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Non-interfering Protein Assay™ kit, Calbiochem®

Supplier: Merck Millipore (Calbiochem‎)

Non-Interfering Protein Assay™ Kit is an easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed.

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Protein Assay, Protn-Latex™

Protein Assay, Protn-Latex™

Supplier: G-Biosciences

A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.

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Quantitative colorimetric peptide assay, Pierce™

Quantitative colorimetric peptide assay, Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.

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Streptavidin poly-HRP conjugate, Pierce™

Streptavidin poly-HRP conjugate, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.

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Glutathione Fluorometric Assay Kit

Glutathione Fluorometric Assay Kit

Supplier: Abcam

Simple assay to detect total glutathione.

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Biotin labelling kit

Biotin labelling kit

Supplier: Abnova

Biotin Labelling Kit is used for simple and rapid preparation of biotin-labeled antibodies and proteins.

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2-D Quant kit

2-D Quant kit

Supplier: Cytiva

2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.

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Histone, from calf thymus, MP Biomedicals

Supplier: MP Biomedicals

Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.

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