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Stains

Stains

Overcome normal optical limitations in microscopic studies by highlighting specific structures with stains and reagents and then protect samples with fixatives. The fast-acting dyes create strong contrasts between cell components for easier detection or analyses. Sensitive attractions to certain organelle or cellular components differ among offered stains, allowing precise testing modifications. Flexible in use, ready-to-use stains may be handled manually or in conjunction with automatic equipment.

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Non-aqueous DPX mounting medium

Supplier: Merck

DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.

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Sigma-Aldrich® Bouin's solution

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

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Neutral mounting medium, DPX, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.

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Aqueous mounting medium, Fluoroshield™ with DAPI, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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Differentiation Solution

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

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Mountant for histology, DPX, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

DPX Mountant for histology has been used as a slide mountant to fix sections.

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Eukitt® mounting medium

Supplier: SIGMA ALDRICH MICROSCOPY

This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.

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Aqueous mounting medium, Fluoromount™, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

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M-FIX™ Spray fixative for gynecological and clinical cytology, Sigma-Aldrich®

M-FIX™ Spray fixative for gynecological and clinical cytology, Sigma-Aldrich®

Supplier: Merck

M-FIX is a fixative spray designed for the wet fixation of gynaecological and non-gynaecological preparations processed in Papanicolaou staining. It contains polyglycols in alcoholic solution and a rapid and secure fixation of specimens is possible.

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Hartman’s fixative histological tissue fixative, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

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Embedding kit JB-4, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

JB-4 is a water-soluble, GMA based, plastic resin kit intended for use in the preparation of embedded samples for high resolution light microscopy. It is a technique now widely used for research and clinical diagnosis. JB-4 yields semi-thin sections (0.5μ-2μ) with excellent morphological preservation.

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Aqueous mounting medium, Fluoroshield™ with PPD, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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Epoxy embedding medium, hardener MNA, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator

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Araldite® M, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.

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ImmunoHistoMount™ Mounting Medium

Supplier: SIGMA ALDRICH MICROSCOPY

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.

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Embedding medium, PolyFreeze, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

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