You searched for: Protein Linking and Labeling Systems
Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.
Pierce™ PEGylated SMCC Crosslinkers, Heterobifunctional
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.
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PSA™ iFluor® 488 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 488 PSA kit is a much superior replacement for Alexa Fluor 488 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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PSA™ iFluor® 555 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 555 PSA kit is a much superior replacement for Alexa Fluor 555 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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PSA™ iFluor® 647 Imaging Kit with Goat Anti-Rabbit IgG
Supplier: AAT BIOQUEST
iFluor® 647 PSA kit is a much superior replacement for Alexa Fluor 647 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.
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Amine reactive biotinylation kits, EZ-Link™
Supplier: Thermo Fisher Scientific
The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.
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Speciality dyes, DyLight™
Supplier: Thermo Fisher Scientific
The DyLight™ Specialty Dyes are fluorescent dyes with varying spectral characteristics, degrees of sulfonation, charges and hydrophobicities. These dyes are offered in their amine-reactive forms and are mainly derivatized with N-hydroxysuccinimide (NHS) esters, the simplest and most commonly used reactive group for labeling proteins. The large selection of near infrared (NIR) and infrared (IR) dyes may be used for in vivo and near-IR fluorescence (NIRF) applications.
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NHS ester quenchers, DyLight™
Supplier: Thermo Fisher Scientific
The DyLight™ NHS Ester Quenchers are amine-reactive non-fluorescent dyes that use fluorescent resonance energy transfer (FRET) to quench fluorescent signals resulting from ultraviolet, visible and infrared light-excited fluorescent dyes. These dyes can be efficiently paired with many DyLight™ dyes and other common fluorescent dyes including fluorescein, rhodamine, cyanine and Texas Red® dye.
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Antibody labelling kits, Mix-n-Stain™ DNP
Supplier: Biotium
Mix-n-Stain™ DNP antibody labelling kits utilise a technology that allows rapid antibody labelling with dinitrophenol without a purification step.
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AZOCOLL™ substrate, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions.
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Biotinylated heparin, porcine intestinal mucosa, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
Biotinylated heparin contains 5 − 8 mole-percent biotin. Biotin attachment is through a 12-atom spacer arm containing a cleavable disulfide group.
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Bilirubin conjugate, ditaurate, disodium salt, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
Bilirubin conjugate, ditaurate is suitable as a direct bilirubin standard. It behaves similarly to purified bilirubin glucuronides from human bile in diazo tests.
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Fluor-free acids, DyLight™
Supplier: Thermo Fisher Scientific
DyLight™ fluor-free acids are non-activated forms of selected DyLight™ fluorescent dyes for use as controls and calibration factors in fluorescence imaging applications. These non-activated fluorescent dyes contain the native carboxyl group of the standard dye molecule.
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PEGylated SPDP Crosslinkers, Heterobifunctional, Pierce™
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.
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Antibody labelling kits, Mix-n-Stain™ Digoxigenin
Supplier: Biotium
Mix-n-Stain™ Digoxigenin antibody labelling kits utilise a technology that allows rapid antibody labelling with digoxigenin without a purification step.
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Streptavidin, maleimide-activated
Supplier: Thermo Fisher Scientific
Maleimide-Activated Streptavidin is for directly preparing streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulphhydryl (-SH) group. Streptavidin has been maleimide-activated using Sulpho-SMCC, a heterobifunctional crosslinker that contains an N-hydroxysuccinimide ester and a maleimide group. The activated streptavidin presents an available maleimide group that reacts with sulphhydryl-containing molecules.
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Biotin NTA
Supplier: Biotium
Biotinylated probes for detection of His-tagged proteins immobilised on nitrocellulose membranes.
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Biotin-NHS
Supplier: Merck Millipore (Oncogene)
Biotin-NHS reacts with primary amines to biotinylate proteins under mild conditions. Since it is water-soluble, this compound can be used to biotinylate cell-surface proteins and proteins that are sensitive to organic solvents.
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Peptide coupling kits, HOOK™
Supplier: G-Biosciences
The HOOK™ Peptide Coupling kits allow researchers to attach peptides to a carrier protein of choice using either primary amines or sulfhydryls in their peptide. The HOOK™ Peptide Coupling kits can be supplied with or without carrier proteins.
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Biotin kits, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin Reagent that conjugates to proteins through amines, sulfhydryls, carboxyls or carbohydrates. The amine and sulfhydryl coupling HOOK™ Biotin Reagents couple directly to the protein through their reactive groups, however the carboxyl coupling HOOK™ Biotin Reagents require a carbodiimide crosslinker and the carbohydrate coupling HOOK™ Biotin Reagents require carbohydrate oxidation before coupling.
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FluorSave™ reagent, Calbiochem®
Supplier: Merck Millipore (Calbiochem)
FluorSave™ reagent is an aqueous mounting medium for use in conjunction with fluorescent tracers such as fluorescein, rhodamine and phycobiliprotein probes.
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Ghost Dye™ Red 710
Supplier: Tonbo Biosciences
Ghost Dye Red 710 is an amine reactive viability dye, that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications.
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High sensitivity NeutrAvidin™ protein-HRP conjugate, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ High Sensitivity NeutrAvidin™-HRP Conjugate is a specially prepared peroxidase conjugated form of avidin biotin binding protein that provides signal amplification like poly-HRP and exceptional storage stability.
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Immunostainer enhancer, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Immunostain Enhancer is compatible with fluorescent and chromogenic detection and routinely increases both signal intensity and detection sensitivity.
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VeriFluor™ Far-Red Passive Reference Dye
Supplier: Biotium
A far-red passive reference dye for qPCR that frees up the ROX channel for use with JUN™ probes, EvaRuby™ qPCR dye, and other orange dyes.
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Amersham™ CyDye™ Value Packs (Mono-Reactive Hydrazide), Cytiva
Supplier: Cytiva
Hydrazide esters provide a convenient method for the labelling of free carbonyl groups of glycoproteins and carbohydrates. For maximum convenience, stable active hydrazide esters are supplied and stored as solid material.
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Amersham Cy3 and Cy5 Maleimide Mono-Reactive Dye 5-Packs, Cytiva
Supplier: Cytiva
Mono-functional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.
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Protein labelling Kit, Amersham™ QuickStain
Supplier: Cytiva
The Amersham™ QuickStain Kit contains a Cy™5 fluorophore and labeling buffer for easy detection of proteins in SDSPAGE gels and on Western blotting membranes. The ready to use Cy5 N-hydroxysuccinimide (NHS) ester and Tris labeling buffer ensure robust and consistent labeling for detection of proteins in diverse samples.
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Fluorescent dyes, M series, streptavidin conjugate
Supplier: Abnova
Fluorescent dye, M series, products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. In comparison with phycobiliprotein tandems, Fluorescent dye, M series, possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
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CyDye Antibody Labeling Kits
Supplier: Cytiva
CyDye Fluorescent Dyes are supplied as highly water soluble, reactive NHS esters for easy conjugation to biomolecules containing aliphatic amino groups.
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Reactive dye 5-packs, Amersham CyDye™
Supplier: Cytiva
Mono-reactive dye pack contains derivatised CyDye™ that carries only one reactive group on each dye molecule for accurate labelling of amine groups, for example on antibodies or oligonucleotides.