You searched for: Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

Supplier: VWR Chemicals

Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

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VWR® Ultra Pure Grade, Sm buffer

Supplier: VWR Chemicals

Cloning Buffer, SM BUFFER

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Phosphate Buffer, pH 6.6 at 25 °C

Supplier: SIGMA ALDRICH MICROSCOPY

Phosphate buffer's buffering capacity is pH sensitive and is preferred to be used at physiological pH (7,2 to 7,4).

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Buffer, TE solution (TRIS-EDTA buffer) pH 8,0

Supplier: Rockland Immunochemicals

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

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VWR®, Buffers, Sodium acetate

Supplier: VWR Chemicals

Sodium acetate buffer C, VWR®

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Rockland Immunochemicals

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Permeabilisation, (buffer)

Supplier: ENZO LIFE SCIENCES

Permeabilization, (buffer)

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Fixation, (buffer)

Supplier: ENZO LIFE SCIENCES

Fixation, (buffer)

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VWR®, Tris buffer solution, pH 9 (1 mol/L), ultrapure

Supplier: VWR Chemicals

Cloning Buffer, TRIS buffer solution pH 9 (1 mol/l)

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VWR® Buffer Tablets for Haematology

Supplier: VWR Chemicals

These tablets provide a rapid and convenient method of preparing a buffer solution.

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Hybridisation buffers

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

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VWR®, TE (Tris-EDTA) buffer, pH 8,0 for biotechnology

Supplier: VWR Chemicals

Ready to use Tris-EDTA buffer.

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VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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Fixation/Permeabilisation, (buffer)

Supplier: ENZO LIFE SCIENCES

Fixation/Permeabilization, (buffer)

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: G-Biosciences

10X PBS (Phosphate Buffered Saline) is a 10 fold concentrated, sterile, pH adjusted blend of phosphate buffer and saline solution, which when diluted 1 to 10 in ultrapure water produces a phosphate buffered saline of 8mM NaH2PO4, 150mM NaCl, 3mM KCl, and 2mM KH2PO4 at pH 7.4.

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Buffer, TRIS solution pH 7 (1 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, TRIS buffer solution pH 7 (1 mol/L)

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Buffer, TBS solution 10X concentrate (TRIS buffered saline)

Supplier: G-Biosciences

The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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VWR®, Rapid Transfer buffer 10X

Supplier: VWR Chemicals

Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

Sustainable Options Available

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Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.