You searched for: qPCR/RT-qPCR Enzymes and Kits
qScript™ XLT One-Step RT-qPCR ToughMix®
Supplier: Quantabio
qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.
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Brilliant II probe MM
Supplier: AGILENT
Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
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Brilliant II SYBR® Master mix
Supplier: AGILENT
Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
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Brilliant III Probe MM, Agilent Technologies
Supplier: AGILENT
Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.
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qPCR SuperMix, PerfeCTa® MultiPlex
Supplier: Quantabio
This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.
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AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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PerfeCTa® QPCR mixes, SYBR® green super mixes and fast mixes
Supplier: Quantabio
PerfeCTa® SYBR® Green SuperMixes and FastMixes™ enable efficient, sensitive and precise quantitative PCR with proprietary buffers and SYBR® Green stabilisers that maximise fluorescent signal, PCR efficiency, and reduce primer dimers. These SuperMixes and FastMixes™ have been optimised for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye or fluorescein.
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Brilliant III Ultra-fast probe ROX qPCR master mix
Supplier: AGILENT
High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.
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AMPIGENE® qPCR 1-Step Probe Kit No-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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Taq extender PCR additive
Supplier: AGILENT
Improves reliability and yield of conventional Taq-based PCR amplifications.
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One-Step Fast qRT-PCR kits, qScript™
Supplier: Quantabio
These kits offer a convenient and highly sensitive solution for Real-Time reverse transcriptase PCR (qRT-PCR) of RNA templates using hybridisation probe detection chemistries, such as TaqMan® 5'-hydrolysis probes.
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Viability PCR starter kits
Supplier: Biotium
Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).
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PerfeCTa® MultiPlex qPCR ToughMix®
Supplier: Quantabio
PerfeCTa® MultiPlex qPCR ToughMix® is a 5X concentrated, ready to use reaction cocktail for Real-Time quantitative PCR (qPCR) that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers, probes and templates. The 5X concentrated ToughMix® allows addition of higher amounts of template and improved detection sensitivity with low concentration samples. PerfeCta® MultiPlex qPCR ToughMix® has been optimised to deliver maximum PCR efficiency, sensitivity, and specificity in reduced reaction volumes with fast cycle or conventional PCR cycling protocols.
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Ultra-Plex™ 1-Step ToughMix® (4X)
Supplier: Quantabio
Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.
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qPCR master mixes, Fast Plus EvaGreen®
Supplier: Biotium
A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.
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qPCR master mixes, Forget-Me-Not™ EvaGreen®
Supplier: Biotium
EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.
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One-Step SYBR® Green qRT-PCR, qScript™
Supplier: Quantabio
qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.
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Brilliant III Ultra-Fast SYBR® green ROX QPCR master mix
Supplier: AGILENT
High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.
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AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.
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Q4ever™ Green
Supplier: AAT BIOQUEST
A real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is a popular laboratory technique of molecular biology based on the polymerase chain reaction (PCR).
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JUN Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
JUN dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.
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VIC Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
VIC dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.
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TAQuest™ qPCR Master Mix for TaqMan Probes *Low ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *Low ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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TAQuest™ qPCR Master Mix with Helixyte™ Green *No ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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TAQuest™ qPCR Master Mix for TaqMan Probes *High ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.
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Maxima™ SYBR green/ROX QPCR master mix (2X)
Supplier: Thermo Fisher Scientific
The Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer and is supplemented with ROX passive reference dye. Only template and primers need to be added. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.