You searched for: Extraction Buffers

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

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NP-40 lysis buffer

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer, Thermo Fisher Scientific

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NP-40 lysis buffer with glycerol (2X)

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific

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Triton® X-100 lysis buffer, pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific

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Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

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NP-40 lysis buffer (2X)

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer (2X), Thermo Fisher Scientific

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RIPA buffer with Triton® X-100 (5X)

Supplier: Thermo Fisher Scientific

RIPA buffer with Triton® X-100 (5X), Thermo Fisher Scientific

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MES lysis buffer with Triton® X-100

Supplier: Thermo Fisher Scientific

MES lysis buffer with Triton® X-100, Thermo Fisher Scientific

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PIPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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Lysis buffers, red blood cells

Supplier: G-Biosciences

These DNase- and RNase-free buffers and reagents are designed for high quality research.

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Succinate, 0,2 M buffer solution, pH 5,0

Supplier: Thermo Fisher Scientific

Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific

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Triton® X-100 lysis buffer with glycerol (2X), pH 7,4

Supplier: Thermo Fisher Scientific

Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific

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ACK Lysing Buffer (1x), TheraPEAK™

Supplier: LONZA

Ammonium-Chloride-Potassium (ACK) Lysing Buffer is used to lyse red blood cells in preparations containing white blood cells.

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E. coli lysis buffer

Supplier: Thermo Fisher Scientific

E. coli lysis buffer, Thermo Fisher Scientific

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CSPL buffer

Supplier: OMEGA BIO-TEK

Component of the Mag-Bind® plant DNA 96 plus kit.

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EasySep™ Red Blood Cell Lysis Buffer

Supplier: STEMCELL Technologies

EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.

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Ammonium Chloride Solution

Supplier: STEMCELL Technologies

Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.