Supplier: EDVOTEK

This experiment introduces chromatographic theory and methods of thin layer chromatography (TLC). A mixture of dyes are separated on a cellulose-based TLC plate using different solvent systems.

Supplier: EDVOTEK

This experiment introduces chromatographic separation to the class and shows students how dyes of different colours separate on the basis of their size and shape. Columns may be rinsed and reused.

Supplier: EDVOTEK

Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

Supplier: G-Biosciences

Prepacked purification columns for protein purification. Supplied in packs of five.

Supplier: MACHEREY-NAGEL

Advanced kit for science education.

Supplier: G-Biosciences

The Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic chromatography utilising a hydrophobic enzyme. The use of the enzyme allows purification followed by a simple enzyme assay to detect the fractions that contain the enzyme. This lab activity involves preparation of a crude protein extract and running hydrophobic chromatography to isolate the enzyme.

Supplier: MACHEREY-NAGEL

Advanced kit for science education.

Supplier: G-Biosciences

The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

Supplier: G-Biosciences

The Affinity Chromatography kit teaches the basic principle of affinity chromatography utilising highly specific affinity columns. This lab activity involves preparation of a crude protein extract and running affinity exchange chromatography for isolation of a protein.

Supplier: MACHEREY-NAGEL

An ideal introductory kit for the science education sector. The kit contains all the the chemicals, plates and accessories for students to perform separations of dyes including: anthaquinone dye mixtures, lipophilic dye mixtures and food dye mixtures. The TLC plates are polyester sheet based with either silica, cellulose or alumina as coating.

Supplier: MACHEREY-NAGEL

Materials kit for scientific education.

Supplier: MACHEREY-NAGEL

Advanced kit for science education.

Supplier: G-Biosciences

A hands on lab activity to study immunoaffinity chromatography and use a specific antibody to purify antigenic proteins from complex samples. This technique involves performing a chromatography procedure using antigen or antibody immobilised on a chromatographic resin. The solution containing antigen or antibody is passed through the column, which specifically and efficiently captures antibodies (antigen). The captured molecules are eluted from the column as a pure fraction. In this lab activity, students perform immunoaffinity chromatography and learn how this method is utilised in research laboratories.

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.

Supplier: G-Biosciences

Dialysis is a routinely used technique in research laboratories to 'change' the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as salts. A sample is placed in a bag with a semi-permeable membrane and then placed in the new solution or water. Small molecules, such as salt, can pass through the membrane, moving from an area of high concentration to low concentration. The larger molecules, such as proteins, are retained in the bag. Microdialysis is a modification of dialysis to overcome the problem of dialysing small volumes of precious samples. Microdialysis uses small devices designed for small volumes. This kit allows students to study dialysis using patented microdialysis devices.