Supplier: Thermo Fisher Scientific

Contains a C4 linker which allows for superior alignment of the phenyl ring with aromatic molecules. Get excellent retention and unique selectivity for aromatic and moderately polar analytes with Thermo Scientific™ Hypersil GOLD™ Phenyl reversed phase HPLC columns.

Supplier: VARIAN

Bond Elut™ CH is a mid-polarity sorbent that exhibits unique selectivities for certain analytes. When employed as a non-polar sorbent, CH has the approximate polarity of a C₂ sorbent. Bond Elut™ CH is often a good choice when non-polar sorbents such as C₁₈, C₈ or C₂ do not provide the desired selectivity.

Supplier: Cytiva

A chromatography resin for the purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®, which provides higher affinity, resulting in higher yields and lower loss of valuable target protein.

Supplier: HAMILTON BONADUZ

This anion column line is designed to meet the special requirements of anion analysis. These four columns cover all aspects of standard anion analysis, separation of all 8 common anions in one run. The unique design of the highly cross-linked porous-packing material, and the strong covalent bonded surface chemistry, ensure excellent durability and high separation efficiency. These columns are designed to be used with carbonate-bicarbonate buffer in any concentration or with hydroxide gradient, and many other mobile phases.

Supplier: VARIAN

Bond Elut™ C18 OH is a non-end capped version of the octadecyl bonded phases that enables the silanols on the silica surface to be more active. This low-load C18 has well-controlled silanol activity that permits the fractionation of metabolites and enhances retention of basic compounds compared to an endcapped C18. The pore size makes it a good choice for peptide extraction.

Supplier: Thermo Fisher Scientific

For fast, accurate titer analysis of monoclonal antibodies (MAbs) in harvest cell cultures. This column provides high throughput and accurate analyses through a combination of low backpressure and high efficiency. Its unique format makes automation of loading, binding, elution and collection using biocompatible systems quick and easy. A non-porous, polymeric resin with a hydrophobic divinylbenzene core and a hydrophilic surface optimised for affinity separation makes this column rugged and reproducible.

Supplier: VARIAN

Bond Elut™ C2 is a fairly non-polar sorbent because of the short chain length of the functional group. C2 is often used during the process of method development if analytes are retained too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase for polar interactions.

Supplier: CHIRAL TECHNOLOGIES

CROWNPAK® CR(+) and CR(-) contain a chiral crown ether as a chiral selector, which is coated onto a 5 µm silica support. CROWNPAK® selectors are mainly used to separate chiral amino acids and other small molecules with primary amine groups. The elution order of the enantiomers can be reversed when necessary.

Supplier: Thermo Fisher Scientific

BioRS, strong cation exchange columns designed for monoclonal antibodies (MAbs) and associated variants. The unique non porous pellicular resin provides the resolving power to separate MAb variants that differ by as little as one charged residue. A hydrophilic layer surrounds the polymeric beads and eliminates hydrophobic interactions with the resin, resulting in very efficient peaks. A proprietary grafted cation-exchange surface provides pH selectivity control for high-resolution separations.

Supplier: VARIAN

Bond Elut™ Jr cartridges have top and bottom Luer fittings allowing easy sample processing when used in conjunction with standard SPE cartridges. Bond Elut™ LRC cartridges have a large reservoir above the sorbent bed and are suitable for use on any standard SPE vacuum manifold.

Supplier: Thermo Fisher Scientific

Based on Core Enhanced Technology, using 4 µm solid core particles, these columns allow users of conventional HPLC methods to enjoy performance far beyond that of columns packed with 5, 4 or even 3 μm fully porous particles. Very high separation efficiencies, using standard HPLC instruments and conditions, provide increased peak resolution and lower limits of detection. An ultra-stable packed bed results in exceptionally robust columns that demonstrate excellent retention and response reproducibility.

Supplier: VARIAN

The PLRP-S family of products consists of a range of pore sizes and particle sizes, all with identical chemistry and fundamental adsorptive characteristics. The particles are inherently hydrophobic so there is no bonded phase, alkyl ligand required for reversed-phase separations. This gives a highly reproducible material that is free from silanols and heavy metal ions. Within the extensive product range are columns suitable for analytical separations and preparative purifications.

Supplier: Thermo Fisher Scientific

These columns are designed for ion pairing reversed phase HPLC (IP-RPLC) analysis of aminoglycoside antibioticss, including drug purity characterisation and quantification, therapeutic drug monitoring and residual control testing in meat / poultry. The Acclaim™ AmG C18 stationary phase is based on a proprietary C18 bonding technology that provides extremely high stability towards acidic conditions.

Supplier: Thermo Fisher Scientific

Hypercarb porous graphitic carbon (PGC, Hypercarb) is a unique stationary phase composed of flat sheets of hexagonally arranged carbon atoms with a satisfied valence, as in a very large polynuclear aromatic molecule, it provides extended separation capabilities, enhanced retention of polar compounds and separtion of structurally related analytes with stability at extreme pH and high temperature.

Supplier: Thermo Fisher Scientific

Accucore™ Polar Premium is a rugged amide embedded C18 phase that offers complementary selectivity to conventional C18. Based on Core Enhanced Technology, they provide fast, high resolution separations without the elevated back pressures required by sub-2 µm particles.

Supplier: VARIAN

Bond Elut™ C8 is very similar in properties to C18, but is not as retentive for non-polar compounds, due to its shorter hydrocarbon chain, and therefore reduced carbon loading. C8 is an excellent replacement for C18 when analytes are too strongly retained for effective elution. The potential for polar interactions is somewhat higher than for C18 because there is less coverage of the silica surface. These polar interactions are not, however, a significant property of C8.

Supplier: VARIAN

Bond Elut™ PH is a non-polar bonded silica material which exhibits a different selectivity to alkyl or aliphatic functionalised phases such as C8 or cyclohexyl. The electron density present in the aromatic ring affords an enhancement in the retention of conjugated or aromatic ring-containing analytes due to desirable pi-pi interactions.

Supplier: MACHEREY-NAGEL

State-of-the-art spherical polymer for highest demands in solid phase extraction. PS/DVB copolymer with secondary and tertiary ammonium modification for mixed-mode SPE with weak anion exchange and reversed-phase properties (WAX).

Supplier: VARIAN

A medium polarity sorbent with many uses, Bond Elut™ CN-E is ideal for applications in which extremely non-polar compounds would be irreversibly retained on high carbon load sorbents such as C₈ and C₁₈. This endcapped version of the cyano sorbent is best utilised when extracting analytes from an aqueous matrix.

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ cobalt chromatography cartridges are convenient, ready-to-use pre-packed devices for efficient purification of polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells. His-tagged proteins are purified from total soluble protein extract using a cobalt-charged tetradentate chelator immobilised onto 6% crosslinked agarose.

Supplier: VARIAN

Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation exchange (SCX) and a strong anion exchange (SAX) sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic and neutral analytes from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from bio fluids.

Supplier: VARIAN

Hexafluoroisopropanol (HFIP) is used as a solvent in GPC for the analysis of important industrial polymers such as polyesters, polyamides and polylactide/glycolide copolymers. For greatly improved performance in extremely polar solvents such as HFIP and trifluoroethanol, we have developed novel 'multipore' technology to produce PL HFIPgel, a PS/DVB packing featuring a monodisperse particle size, high pore volume and high resolution.

Supplier: VARIAN

The Bond Elut™ Certify extraction cartridge utilises a packed bed consisting of a special, non-polar C8 sorbent and a strong cation exchanger (SCX). Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for extraction of a wide range of compounds from a diverse range of aqueous matrices.

Supplier: Thermo Fisher Scientific

Pierce™ Glutathione chromatography cartridges are convenient, ready to use pre-packed devices for the purification of GST-fusion proteins from cellular lysates. The glutathione is immobilised through its central sulfhydryl onto 6% crosslinked agarose resin. Purification of GST-fusion proteins using glutathione (GSH) agarose beads is well documented and provides a one-step, high purity affinity purification.

Supplier: VARIAN

Bond Elut™ LMS polymeric sorbent lets you elute without having to add amine modifiers, buffers, or acids. The elimination of secondary interactions means that elution of analytes can be achieved with pure organic solvents or HPLC mobile phase-compatible solvent mixtures of low ionic strength. These characteristics are crucial to allow compatibility with LC/MS or other delicate analytical techniques.

Supplier: Thermo Fisher Scientific

These columns offer unique selectivity for aromatic and moderately polar analytes. Suitable for complex samples where some peaks are well resolved on a phenyl phase whilst others require a conventional alkyl phase. Based on Core Enhanced Technology, they provide fast, high resolution separations without the elevated back pressures required by sub-2 µm particles.

Supplier: VARIAN

Bond Elut™ Diol (2OH) resembles unbonded silica in its tendency for strong hydrogen bonding with analytes. 2OH can also be employed in the non-polar mode because the hydrocarbon spacer on its functional group provides enough non-polar character for retention of hydrophobic analytes. Bond Elut™ Diol is a listed SPE device for the DIN 14333-1 method on benzimidazole fungicides.

Supplier: VARIAN

Bond Elut™ DEA bears some resemblance to Bond Elut™ NH₂ in its properties but with a slightly lower capacity as an anion exchange sorbent. DEA has a moderately non-polar character due to the alkyl side chains on the amino functionality. These groups still afford a medium level of polarity, higher than C₈ but less polar than C₂ or CN-E.

Supplier: Thermo Fisher Scientific

SilTite™ ferrules are designed for use with capillary column connectors.

Supplier: Cytiva

HiLoad™ Superdex™ prep grade are prepacked XK columns designed for pre-parative gel filtration. Superdex™ prep grade is a composite matrix of dextran and highly cross-linked agarose. The steep selectivity of dextran, and the high chemical and physical stability of agarose, enable high resolution separations. The chromatography media combines high mechanical strength with high hydrophilicity, allowing high flow rates and minimal non specific interactions.