Paraformaldehid 4% in PBS ready-to-use, fixative reagent
Supplier: Biotium
Paraformaldehyde, 4% in PBS is a ready-to-use fixation solution for cells or tissues.
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Cell viability assay kits
Supplier: Biotium
MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.
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GelRed™ és GelGreen™ nukleinsav gélfestékek
Supplier: Biotium
A nukleinsav festéséhez szolgáló GelRed és GelGreen fluoreszcens gélfestékek kiemelkedően stabilak és nagy érzékenységűek,ugyanakkor toxicitásuk alacsony.
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Vásárolja meg Acros & Alfa Aesar vegyszereit az Avantor®-tól, hogy optimalizálja laboratóriuma ellátását
Már bízik a Thermo Fisher kiváló minőségű vegyi anyagaiban, valamint az Avantor® kiváló szolgáltatásában és támogatásában. Nos, miért ne kombinálná a kettőt?
PMA™, PMAxx™, Real-Time PCR Viability Kit
Supplier: Biotium
Viability PCR With PMA and PMAxx™ dyes
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EvaGreen® Plus dye, 20X in Water
Supplier: Biotium
EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.
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One-Step Blue® protein gel stain
Supplier: Biotium
One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing.
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Wheat germ agglutinin (WGA) conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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Nucleic acid stains, PAGE GelRed™
Supplier: Biotium
Stains specifically designed for DNA in polyacrylamide gels.
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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™
Supplier: Biotium
EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.
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Fast probe master mixes
Supplier: Biotium
A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.
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Dimetil-szulfoxid, vízmentes ≥99.9%
Supplier: Biotium
Anhydrous DMSO (dimethyl sulfoxide; methyl sulfoxide) is recommended for preparing stock solutions.
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GelRed® Prestain Plus 6X DNA loading dye
Supplier: Biotium
6X DNA loading buffer that includes ultra-sensitive, non-toxic GelRed® dye.
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PMAxx™ dye 20 mM in water
Supplier: Biotium
PMAxx™ dye is a DNA modifier used for viability PCR (v-PCR). V-PCR is a powerful PCR-based, culture-independent, method for rapid and sensitive quantification of microbial viability.
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SYBR® Green I 10000x in DMSO nucleic acid gel stain
Supplier: Biotium
Thiazole green, which is structurally identical to SYBR® green I nucleic acid gel stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.
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Super HT PAP pens
Supplier: Biotium
The Super HT PAP pen draws a water repellent circle around slide mounted tissue and prevents the waste of valuable reagents by keeping the liquid pooled in a single droplet. The regular sized super HT PAP pen can be used in about 800 applications, while the mini sized super HT PAP Pen will last about 400 applications.
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Propidium iodide 1 mg/ml in water
Supplier: Biotium
Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.
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6X GelRed prestain loading buffer with tracking dye
Supplier: Biotium
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).
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Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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PMA (Propidium monoazide) DNA/RNA binding dye
Supplier: Biotium
PMA (propidium monoazide) is a high-affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids.
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Lipofuscin autofluorescence quencher, TrueBlack™
Supplier: Biotium
TrueBlack™ is a reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy and accumulate in a cell and tissue types with age.
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PMA (Propidium monoazide) 20 mM in water DNA/RNA binding dye
Supplier: Biotium
PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA.
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qPCR master mixes, Forget-Me-Not™ EvaGreen®
Supplier: Biotium
EvaGreen® Dye and high-performance dye-based qPCR master mixes containing EvaGreen® qPCR dye, Cheetah™ HotStart Taq Polymerase, and Forget-Me-Not™ tracking dye. Available with 2-colour tracking or pre-mixed with low or high ROX.
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Cell scrapers
Supplier: Biotium
PE, disposable, sterile. Mini cell scrapers for harvesting cells or cell lysates from microwell plates.
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dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range
Supplier: Biotium
AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.
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Tetramethylrhodamine ethyl ester perchlorate (TMRE) ≥95% (by HPLC) mitochondria dye, suitable for fluorescence
Supplier: Biotium
TMRE (Tetramethylrhodamine ethyl ester, perchlorate) and TMRM (70017) are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation.
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5(6)-Carboxy-2′,7′- dichlorofluorescein diacetate mixture of isomers ≥98% (by TLC) fluorescent pH indicator
Supplier: Biotium
5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate is membrane-permeant and thus can be loaded into cells via incubation. The diacetate is readily hydrolyzed to 5-(and-6)-carboxy-2′,7′-dichlorofluorescein (51015) by intracellular esterases.
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5(6)-ROX N-succinimidyl ester amine-reactive fluorescent dye
Supplier: Biotium
5(6)-ROX, SE (full name: 5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester) is an amine-reactive long wavelength rhodamine dye.
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5(6)-Carboxyrhodamine 110-X hydrochloride ≥95% (by TLC) amine-reactive fluorescent dye
Supplier: Biotium
5-(and-6)-Carboxyrhodamine 110-X (full name: 5-(and-6)-Carboxyrhodamine 110-X, hydrochloride) is similar to 5-(and-6)-carboxyrhodamine 110 (#90000). The X linker molecule is useful to reduce the interaction between the dye and the molecule to be labeled.
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Reactive dye conjugates
Supplier: Biotium
Reactive dyes containing an amine functional group.
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FITC (fluorescein-5-isothiocyanate, fluorescein isothiocyanate isomer I) ≥98% (by TLC) amine-reactive fluorescent dye
Supplier: Biotium
FITC (fluorescein-5-isothiocyanate) is an amine-reactive fluorescein derivative for labeling proteins or other molecules containing primary amines.