Clostridium difficile Toxin A + Toxin B ELISA kit is a single-wash 90-min Simplestep used to quantify Clostridium difficile Toxin A + Toxin B with a sensitivity of 37,638 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader.