Human recombinant Aldehyde dehydrogenase 1-A1 (from E. coli)
PRSI92-097
:
- Pk:50 µG
- Enzyme type:Recombinant
- Source:E. coli
- Species:Human
- Tag sequence:N-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:Aldehyde dehydrogenase 1-A1
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:57 kD
- Sequence:Met1-Ser501
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH₂O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
Aldehyde Dehydrogenase Family 1 Member A1 (ALDH1A1) is a cytoplasmic enzyme that belongs to the Aldehyde Dehydrogenase family. ALDH1A1 is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties and subcellular localisations. ALDH1A1 is the main cytosolic isoform, which has a lower affinity for aldehydes than the mitochondrial enzyme. ALDH1A1 binds free retinal and cellular retinol-binding protein-bound retinal. It can convert/oxidise retinaldehyde to retinoic acid.
Fusion-Tag: N-6 His tag
This recombinant protein can be used for biological assays. For research use only.