Human recombinant Phosphomannomutase 2 (from E. coli)

PRSI91-230

Human recombinant Phosphomannomutase 2 (from E. coli)

PRSI91-230

Pk:
50 µG
Enzyme type:
Recombinant
Source:
E. coli
Species:
Human
Tag sequence:
C-6 His tag
Storage conditions:
Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
Enzyme name:
Phosphomannomutase 2
Purity:
>95% as determined by reducing SDS-PAGE
Molecular weight:
29.1 kD
Sequence:
Met1-Ser246
Formulation:
Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested applications:
Bioassay

Phosphomannomutase 2 (PMM2) is an enzyme that is a member of the highly variable methyltransferase superfamily. PMM2 is a cytoplasmic protein and catalyses the isomerisaion of mannose 6-phosphate to mannose 1-phosphate.In addition, PMM2 involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose that required for a number of critical mannosyl transfer reactions. Defects in PMM2 can results in congenital disorder of glycosylation type 1A (CDG1A). Congenital disorders of glycosylation are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation.

Fusion-Tag: C-6 His tag

This recombinant protein can be used for biological assays. For research use only.