E. coli

Rat

Lyophilized Leptin Antagonist Triple Mutant Rat Recombinant although stable at room temperature for several weeks, should be stored desiccated below −18 °C. Upon reconstitution at 0. 1 Leptin mutant mg/ml and up to mM and filter sterilization LEP mutant can be stored at 4 °C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of carrier protein (0. 1% HSA or BSA) is suggested. Please prevent freeze/thaw cycles.

Leptin Antagonist Triple Mutant Rat Recombinant half-life in circulation after SC injection was over 20 hours.Leptin Antagonist Triple Mutant Rat Recombinant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Leptin Antagonist Triple Mutant Rat Recombinant in vitro activity is 5-6 fold lower than the non-pegylated antagonist, though in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist), resulting mainly from increased food intake.

LEP

Leptin Triple Antagonist, Pegylated

Greater than 99.0% as determined by(a) Gel filtration analysis.(b) Analysis by SDS-PAGE.

It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Rat Recombinant in sterile water or sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.

The The Rat Leptin triple anatagonist was lyophilized from concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.

SDS-PAGE