Specifications

• Protein/peptide type:
  Recombinant
• Source:
  E. coli
• Species:
  Mouse
• Storage conditions:
  Lyophilized pegylated murine IL22 although stable at room temperature for several days, should be stored desiccated below −20 °C. Upon reconstitution at 0. 1mg/ml pegylated mouse IL22 and up to 2mg/ml, filter and sterilized, the protein can be stored at °C Celsius for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of carrier protein (0. 1% HSA or BSA) is suggested. Please prevent freeze/thaw cycles.
• Biological activity:
  The ED50 as determined by STAT3 phosphorylation assay in HepG cells. The activity in vitro was found to be 10% compared to the non-pegylated mouse IL22.
• Protein synonyms:
  IL-TIF
• Protein/peptide name:
  mIL 22 PEG
• Purity:
  Greater than 98.0% as determined by: (a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE.
• Solubility:
  It is recommended to reconstitute the lyophilized pegylated mouse Interleukin -22 in sterile 0.4% NaHCO3 adjusted to pH 8-9 not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Formulation:
  Lyophilized from concentrated solution at 0.65mg/ml containing 0.003mM NaHCO3.
• Tested applications:
  SDS-PAGE