Specifications

• Protein/peptide type:
  Recombinant
• Source:
  E. coli
• Species:
  Bacteria
• Storage conditions:
  Lyophilized Bacterial Outer Membrane Protein-A although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon between 2-7 days and for future usereconstitution OmpA should be stored at below −18 °C. For long term storage it is recommended to add carrier protein (0. 1% HSA or BSA). Please avoid freeze/thaw cycles.
• Biological activity:
  The interaction of bacterial and recombinant A-layer protein with murine macrophages was directed at determining the effect of A-protein on intracellular events that occur in primed macrophages. This was accomplished by measuring the cytotoxic product produced by peritoneal macrophages when exposed to A-protein coated latex beads. Thioglycolate elicited macrophages exhibited low level of activation (18% cytotoxicity) that was significantly increased (48% cytotoxicity) in the presence of latex beads. Coating of the latex beads with each of the three A-protein products resulted in an increase of cytoxicity (mean +/- SEM) from 48% to 91%.
• Protein synonyms:
  Outer Membrane
• Protein/peptide name:
  OmpA
• Purity:
  Greater than 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Solubility:
  It is recommended to reconstitute the lyophilized OmpA in sterile 0.4% NaHCO3.
• Sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Val-Val-Ile-Ser.
• Tested applications:
  SDS-PAGE